Propionic acid bacteria. Olivia Rasigraf HMC 2012. General. Gram + , non- sporeforming Facultative anaerobes High GC Secondary fermenters Contain cytochromes Habitats: skin, cheese. Isolation. Lactate-yeast extract broth. Incubation at 30°C, dark. “ Emmentaler ” habitat. CO 2.
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Lactate-yeast extract broth
Incubation at 30°C, dark
3 Lactate → 2 Propionate + 1 Acetate + 1 CO2 + 1 ATP
Methylmalonyl-CoA mutase (B12)
Brown colonies: Methylmalonyl-CoA pathway
White colonies: Acrylyl pathway