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Study on the Tgt1 transposon system with XbaI and EcoRI restriction sites, 2180bp LTR elements, ORF1 and ORF2, intron, and exon1/exon2 sequences to assess its gene insertion efficiency and potential applications.
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(a) Tgt1 (9,446bp) Xba I Xba I Eco RI Eco RI PBS PPT LTR LTR 2180bp 2180bp AGCAG AGCAG ORF1 (488aa) ORF2 (1152aa) intron f3′5′h2Tgt1 exon1 exon2 (b) 1 2 1 2 (kbp) 10- 6- 4- 3- 2- 1- 1h 10h Exposure time Fig. S5