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ADULT

Source: Color Atlas of Medicine and Parasitology. 1977 W. Peters & H.M. Gillers. Wucher er ia bancrofti. MORPHOLOGY. ADULT. MALE 40 x 0,1 mm, yellowish white, cylinder, slender, Caudal part bends to ventral portion.

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ADULT

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  1. Source: Color Atlas of Medicine and Parasitology. 1977 W. Peters & H.M. Gillers Wuchereriabancrofti MORPHOLOGY ADULT • MALE • 40 x 0,1 mm, yellowish white, cylinder, slender, • Caudal part bends to ventral portion

  2. Sumber : Color Atlas of Medicine and Parasitology. 1977. W. Peters & H.M. Gillers Wuchereria bancrofti MORPHOLOGY ADULT • FEMALE • (80-100) x (0,24-0,30) mm, soft, blunt nail, slight rounded head.

  3. Cephalic space Sheath Sheath Slender tail Nuclei MORPHOLOGY OF MICROFILARIAE Source : Medical Parasitology in Plates. G. Piekarski

  4. Microfilaria Brugia malayi Microfilaria Wuchereria bancrofti Microfilaria Brugia timori

  5. Cephalic space (L:W=1:1) Tail (No nuclei) Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk. Pewarnaan hematoxylin Source : Color Atlas of Medicine and Parasitology. 1977 W. Peters & H.M. Gillers. Pewarnaan giemsa Wucheria bancrofti MORPHOLOGY MICROFILARIAE

  6. Cephalic space (L:W=2:1) B. malayi W. bancrofti Bloated tail (nucleus in it) Brugia malayi MORPHOLOGY MICROFILARIAE

  7. Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk. Brugia timori MORPHOLOGY MICROFILARIAE • Looking like microfilariae B. malayi • Size 287-341  • Sheathed not clear at coloration of Giemsa (dr. Djaenudin Natadisastra, SpParK)

  8. Source : Atlas of Medical Parasitology. Prayong Radomyos, dkk. Brugia timori MORPHOLOGY MICROFILARIAE • Stiff body broken, tip of tail rather blunt • “Nuclei” in the body, spread in the irregular groups, presence of (5-8) nuclei in the tail • Smaller nuclei at the distal than in B. malayi • Cephalic space: 3 : 1

  9. GENERAL MORPHOLOGY Plasmodium sp. IN ERYTHOCYTIC CYCLES • FOUND TWO MAIN STAGE : • TROPHOZOITE STAGE • Ring form • Old/Matured trophozoite • SCHIZONT STAGE • Young schizont • Old/Matured schizont

  10. Protoplasm Nucleus ERYTHROCYTE • Ring Form with reddish Nucleus and bluish Protoplasm • Chromatin dots Plasmodium sp. MORPHOLOGY TROPHOZOITE STAGE

  11. Protoplasm Nucleus ERYTHROCYTE Nucleus and Protoplasm grow bigger Plasmodium sp. MORPHOLOGY TROPHOZOITE STAGE

  12. Protoplasm Nucleus ERYTHROCYTE • Nucleus starts fission process • Protoplasm remains in size Plasmodium sp. MORPHOLOGY SCHIZONT STAGE

  13. Merozoite ERYTHROCYTE • Complete Merozoites • Note the Regularity, Pigment Plasmodium sp. MORPHOLOGY SCHIZONT STAGE

  14. ERYHTROCYTE Ring Form ERYTHROCYTE • Ring Form • Erythrocyte enlarged, pale • Schuffner dots PLASMODIUM VIVAX TROPHOZOITE STAGE

  15. ERYTHROCYTE ERYTHROCYTE Amoeboid Form Protoplasm grows (Amoeboid form) with vacuole PLASMODIUM VIVAX TROPHOZOITE STAGE Vacuole

  16. Fission of Nucleus ERYTHROCYTE • Fission of Nucleus • Vacuole disappear PLASMODIUM VIVAX SCHIZONT STAGE

  17. Pigment MEROZOITe • Merozoite (12-14) • Pigment PLASMODIUM VIVAX SCHIZONT STAGE

  18. Pigment NUCLEUS NUCLEUS • Round/Oval • Small nucleus , compact, eccentric • Bluish protoplasm • Round/Oval • Big nucleus, pale • Red-blue protoplasm PLASMODIUM VIVAX GAMOTOCYTE STAGE: Macro and Microgametocyte

  19. PLASMODIUM OVALE TROPHOZOITE STAGE • Ring Form (thick) • Erythrocyte • Chromatin dots

  20. PLASMODIUM OVALE TROPHOZOITE STAGE Round, compact trophozoite Erythrocyte deformed Schuffner/James’ Dots

  21. Resemble P. vivax Normal erythrocyte Plasmodium malariae TROPHOZOITE STAGE

  22. Trophozoite Band form Trophozoite Plasmodium malariae TROPHOZOITE STAGE

  23. Pigment MEROZOITE • Rossete-like • Merozoit (6-12) Plasmodium malariae SCHIZONT STAGE

  24. Round/Oval • Large nucleus, pale • Reddish protoplasm • Round/Oval • Small nucleus, compact • Bluish protoplasm Plasmodium malariae GAMETOCYTE STAGE: Macro and Microgametocyte

  25. TROPHOZOITE STAGE Plasmodium falciparum Fine ring Multiple infection

  26. Vacuole • Vacuole surrounded by plasma • Maurer’s dots Plasmodium falciparum TROPHOZOITE STAGE

  27. Schizont occupy 2/3 erythrocyte • 8-24 Merozoite Plasmodium falciparum SCHIZONT STAGE

  28. Plasmodium falciparum GAMETOCYTE STAGE: Macro and Microgametocyte • Sausage shape • Large nucleus, pale, more eccentric position • Pigment dispersed • Reddish protoplasm • Banana/crescent shape • Small nucleus, compact, in the middle • Pigment around nucleus • Bluish protoplasm

  29. THICK BLOOD PREPARATION Plasmodium falciparum PLASMODIUM VIVAX • Uniform • No red zone around parasite • Smaller than lymphocyte nucleus Plasmodium malariae • Not uniform • No red zone around parasite • Smaller than lymphocyte nucleus • Not uniform • Red zone around parasite • Bigger than lymphocyte nucleus

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