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电渗是 CE 的基本现象之一，它可以控制组分的迁移速度和方向，进 而影响 CE 的分离效率和重现性．

电渗是 CE 的基本现象之一，它可以控制组分的迁移速度和方向，进 而影响 CE 的分离效率和重现性．. 如何控制电渗流？. 理论： 能影响电渗的因素都有可能用于电渗的控制 实际： 能理想地调节电渗而又不影响分离过程的控制方法目前 不多见． 对于 DNA 分离及测序来说， pH-8.3 ，常用管壁涂层（涂覆 ) 技术. 化学涂覆. 如聚丙烯酰胺、聚乙烯基吡咯烷酮涂层可在 pH9-pH6 以内基本消除电渗. 优点. 抑制熔硅毛细管表面 Si － OH 解离，修饰表面 Zeta 电势． 聚合物或硅烷化试剂的取代基覆盖已离解的 Si － OH ．

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电渗是 CE 的基本现象之一，它可以控制组分的迁移速度和方向，进 而影响 CE 的分离效率和重现性．

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  1. 电渗是CE的基本现象之一，它可以控制组分的迁移速度和方向，进电渗是CE的基本现象之一，它可以控制组分的迁移速度和方向，进 而影响CE的分离效率和重现性．

  2. 如何控制电渗流？ 理论： 能影响电渗的因素都有可能用于电渗的控制 实际： 能理想地调节电渗而又不影响分离过程的控制方法目前 不多见． 对于DNA分离及测序来说，pH-8.3，常用管壁涂层（涂覆) 技术.

  3. 化学涂覆 如聚丙烯酰胺、聚乙烯基吡咯烷酮涂层可在pH9-pH6以内基本消除电渗

  4. 优点 • 抑制熔硅毛细管表面Si－OH解离，修饰表面Zeta电势． • 聚合物或硅烷化试剂的取代基覆盖已离解的Si－OH． • 增加了内壁表面层附近溶液的粘度，涂层的厚度和密度都将影响到表面　　　层附近溶液的粘度，从而影响涂层的涂覆效果。 • 涂层寿命长，稳定性好，分离效率高。 缺点 • 化学键合的过程中涉及毛细管硅烷化和引发单体聚合两步反应，一方面受两步反应的转化率影响，Si－OH很难被完全屏蔽；另一方面，毛细管内的聚合反应很难控制，容易造成涂层的不均一性、不规整性，甚至阻塞毛细管。 • 价格昂贵。

  5. 物理涂覆

  6. 优点 • 涂层制备过程简单易操作，涂覆过程可一步完成， • 涂层可再生。这种涂层可以是中性的、带正电荷的、也可以是吸附在带正电的聚合物涂层表面转而带负电荷的。 缺点 物理吸附涂层稳定性不及化学键合涂层．

  7. 用于DNA分离的聚合物 非交联聚合物 交联聚合物: 交联聚丙烯酰胺、琼脂糖

  8. 均聚物 poly-N-hydroxyethylacrylamide（PHEA) 存在的问题：

  9. 共聚物 理想的筛分介质：高筛分能力、低粘度、自涂覆功能 a. 无规共聚物 poly (DEA-co-DMA)　 聚 (N, N -二乙基丙烯酰胺- co -N, N - 二甲基丙烯酰胺） poly (AM-co-DMA)　 聚(丙烯酰胺- co - N, N - 二甲基丙烯酰胺) poly (AA-co-AG)　　 聚(丙烯酰胺- co - β-吡喃型葡萄糖) poly(AM-co-AAG)　　 聚(丙烯酰胺- co -葡糖酰丙胺) poly(NEEA-co-NMEA)　聚(乙氧基乙基丙烯酰胺- co -乙氧基甲基丙烯酰胺)等 b.嵌段共聚物 PEG-(CnF2n+1)2 末端带有碳氟化合物的聚乙二醇 PEO-PPO-PEO 低分子量的聚环氧乙烷和聚环氧丙烷的三嵌段共聚物 BEB/EBE 聚环氧乙烷和聚环氧丁烷的三嵌段共聚物等

  10. C. 接枝共聚物 PAM-g-PNIPAM （聚丙烯酰胺-g- 聚N-异丙基丙烯酰胺) PNIPAM-g-PEO (聚N-异丙基丙烯酰胺-g- 聚环氧乙烷) PAM-g-PDMA （聚丙烯酰胺 -g- 聚N，N- 二甲基丙烯酰胺） PDMA-g-PMMA（聚N，N- 二甲基丙烯酰胺-g-聚甲基丙烯酸甲酯）等 存在的问题：

  11. 共混聚合物 • 不同分子量的PEO分离ds 和ss DNA • HEC，LPA等不同分子量的混合物也都能成功地对DNA分离或排序 存在的问题：

  12. 准互穿网络聚合物

  13. Yanmei Wang,Dehai Liang, Jingcheng Hao, Dufei Fang, Benjamin Chu, Electrophoresis 2002, 23, 1460-1466

  14. Mechanism of particle formation by inverse microemulsion polymerization of AM/AIBN system. M is monomer acrylamide; I is initiator; R is free radical.

  15. Electrophoretic separation of DNA fragments generated on Big Dye TM Terminator Cycle Sequencing Standard with AmpliTaq FS under optimum experimental conditions: 2.5% IPNs, 200 V/cm and room temperature. A-track red, T-track blue, G-track green, and C-track black. Electrophoretic conditions were as follows: effective length 36 cm, total length 41cm, 75-m-i.d., 365-m-o.d. anode buffer 1TTE; cathode buffer 1TTE/7M urea. The sample was injected at a constant electric field of 73 V/cm for 40 s. Yanmei Wang, Dehai Liang, Q. Ying, B. Chu，Electrophoresis 2005, 26, 126-136

  16. initiator quasi-IPN LPA DMA GNPs quasi-IPN/GNPs quasi-IPN quasi-IPN/GNPs复合介质 Preparation of quasi-IPN/GNPs composite matrices

  17. During the preparation of Au colloids, citrate acted as both a reducing agent and a capping agent to avoid aggregation of GNPs. The particle sizes of prepared GNPs could be controlled by the amount of trisodium citrate. TEM images of (A) GNPs20 (~20 nm), (B) GNPs40 (~40 nm), and (C) GNPs60 (~60 nm) in water, and (D) GNPs40 (~40 nm) in quasi-IPN3 polymer solution.

  18. DNA sequencing by CE and data analysis The full four-color (base C, T, A, G) electropherograms of Bigdye Terminator V 3.1 sequencing standard DNA sample by CE using 2.5% w/v quasi-IPN3/GNPs40-1 at 50℃. Sequencing conditions: effective/total length of bare fused-silica capillaries, 50/61 cm; id/od, 75/365μm; sequencing electric field strength, 150 V/cm; DNA electrokinetic injection, 41 V/cm for 30 s; anode buffer, 1×TTE; cathode buffer, 1×TTE/7M urea.

  19. The partial four-color (base C, T, A, G) electropherograms of standard DNA sample using 2.5% w/v quasi-IPN3/GNPs40-1 by CE at 50℃. Electrophoresis, 2007, 28, 1072-1080. Electrophoresis, 2007, 28, 2998-3007 • 中国发明专利：申请号：200610114099.3. 公开号：CN1966719A

  20. quasi-IPN/functionalized multi-walled carbon nanotubes Schematic representation of preparation of MWNT-COOH, MWNT-Br and MWNT-PDMA via ATRP Schematic representation of the formation of quasi-IPN/MWNT-PDMA double-network composite sieving matrix. Resolution vs. base number in DNA sequencing by CE using quasi-IPN Electrophoresis, 2008, 29, 4637-4645. TEM images of (A) crude MWNTs in water and (B) MWNT-PDMA in quasi-IPN solution.

  21. ■ HEC ○ HEC-g-PAM-1 ▲ HEC-g-PAM-2 ◇ HEC-g-PAM-3 ▼ HEC-g-PAM-4 HEC-g-PAM Formation of HEC-g-PAM Viscosity versustemperature for different HEC-g-PAM copolymers. .Resolution versus size of DNA fragments by using HEC, HEC-g-PAM and PDMA as separation medium at 1.5% w/v. Schematic representation of the structure of HEC-g-PAM copolymer and of its adsorption to a capillary wall. Electrophoresis, 2007, 28, 3223-3231.

  22. 价格表

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