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Gene silencing in plants. - PowerPoint PPT Presentation


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Transcription. Transcription. Poly A. Poly A. Poly A. GFP. GFP. GFP. intron. PFG. gene silencing mRNA degradation. Translation. DICER. GFP. RISC. PFG. Poly A. CAP. CAP. CAP. CAP. CAP. Poly A. DICER. GFP. p 35S. p 35S. GFP. intron. PFG. No Fluorescence. Fluorescence.

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gene silencing in plants

Transcription

Transcription

Poly A

Poly A

Poly A

GFP

GFP

GFP

intron

PFG

gene silencing

mRNA degradation

Translation

DICER

GFP

RISC

PFG

Poly A

CAP

CAP

CAP

CAP

CAP

Poly A

DICER

GFP

p 35S

p 35S

GFP

intron

PFG

No Fluorescence

Fluorescence

LB

RB

LB

RB

RISC

Gene silencing in plants.
1 a gene silencing of transiently expressed sequences

UV

Nb

GFP

GFP

p 35S

p 35S

GFP

intron

p 35S

PFG

LB

LB

RB

RB

LB

RB

1.a. Gene silencing of transiently expressedsequences.
1 b triggering gene silencing of established transgene by transient expression

UV

GFP

intron

p 35S

PFG

LB

RB

GFP

1.b. Triggering gene silencing of established transgene by transient expression.
1 c virus induced gene silencing vigs

Nt

Nt

Nt

RZ

RZ

16K

Agroinfiltration

PTGS PDS

CP

MP

pTRV1

RpRd

p 35S

Sistemic PTGS

pTRV2

RB

LB

LB

RB

PDS

p 35S

1.c. Virus-induced gene silencing (VIGS)
2 systemic gene silencing

UV

GFP

GFP

intron

p 35S

PFG

LB

RB

GFP

2. Systemic gene silencing.

GFP

Agroinfiltration

Sistemic PTGS

PTGS GFP

3 a suppressing gene silencing of transiently expressed sequences

HcPro

p 35S

UV

Nb

GFP

GFP

GFP

p 35S

p 35S

p 35S

GFP

GFP

intron

intron

p 35S

p 35S

PFG

PFG

LB

RB

RB

LB

LB

RB

RB

LB

LB

RB

LB

RB

3.a. Suppressing gene silencing of transiently expressed sequences.
slide7

HcPro

p 35S

UV

GFP

GFP

intron

intron

p 35S

p 35S

PFG

PFG

RB

LB

LB

RB

LB

RB

GFP

3.b.Silencing of trangenes or endogenous genes cannot be established in the presence of silencing suppressor.
3 c suppression of gene silencing mediated by virus infection

PVY infection.

Plant silenced for the Magnesium chelatase gene.

Plant silenced for the Magnesium chelatase gene.

3.c. Suppression of gene silencing mediated by virus infection.