The Role of SMRT in Transcriptional Regulation During Neural Stem Cell Development
This presentation examines the critical role of SMRT (NcoR2) as a nuclear co-repressor in the transcriptional regulation of neural stem cells. Focusing on the methylation of histones at lysine 27 and the action of demethylases, we explore how these processes influence cell fate during neurogenesis. Key visuals will illustrate the impact of SMRT deficiency in neural development, including insights from in situ hybridization and immunofluorescence studies. The goal is to engage a broad audience by emphasizing the significance of transcriptional regulation in nervous system development and regeneration.
The Role of SMRT in Transcriptional Regulation During Neural Stem Cell Development
E N D
Presentation Transcript
Clear Interesting Oral presentation Lab meeting Journal club Qual Thesis Presentation at meeting
Choice of a paper Goal: 1. On topic 2. Important and interesting to a large audience Localization AND(Cell [ta]OR Science [ta]OR Nature [ta]) Nature Methods
Introduction 2-3 slides From general to particular
Significance? Question? What do I need to know? Scheme/visuals? Punch line? Introduction-Example 1 • Transcription is important in neural stem cells • SMRT=NcoR2 is a nuclear co-repressor • Histones get methylated at lysine 27. • There are demethylases that target these lysines • SMRT -/- mice have defects in the cortex.
Neural stem cells neural stem cells progenitors neuronal glial Development of the nervous system Regeneration of adult brain
Transcriptional regulation in neurogenesis? SMRT Program of target genes? Neuronal fate
Localization of molecules In situ hybridization of RNA: Target genes for SMRT Immunofluorescence:Role of SMRT on cell fate (neuronal versus glial markers)
Approaches 1-2 slides 1 scheme is better than 1000 words Emphasize controls and limitations
Title? Method used? Legend? Are all panels relevant? Is the Figure relevant? Conclusion? Example-Figure1
WT KO WT KO Emx2 Mash1 Dlx1 Gad1 SMRT normally prevents neuronal differentiation Method: In situ hybridization on brain sections at embryonic Day 12.5 Markers of differentiated neurons were increased in SMRT KO brains
Black letters Yellow letters Packaging Use Arial or Helvetica bold Front 24-44 Use animation *not too much*
Delivery Stand up Look at the audience Speak loudly + articulate Display enthusiasm Practice makes perfect
