Biochemical instrumental analysis-3. Dr. Maha Al- Sedik. Emission spectroscopy. LUMINESCENCE. Florescence : The light emission of a given wavelength by a substance that had absorbed light of a different wavelength. OR
Dr. Maha Al-Sedik
Fluorescence occurs when an orbital electron of a molecule, atom or nanostructure relaxes to its ground state by emitting a photon of light after being excited to a higher quantum state by some type of energy.
I am the florescent substance .I take what you can not see and give you what you can see
The most striking examples of fluorescence occur when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible to the human eye, and the emitted light is in the visible region..
Labeling of antigen with fluorescence label:
Antibody to these antigen are labeled with fluorescence so allowing its binding to the Ag.
When a fluorescent dye is conjugated to a monoclonal antibody, it can be used to identify a particular cell type based on the individual antigenic surface markers of the cell.
what happens to the laser light as it strikes the single-file particles.