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Analysis of Cross Amplification Overlaps in BAC Clones

This study presents a comprehensive working map illustrating the overlaps in cross amplification among selected BAC clones. The map features vertical arrows that indicate successful amplification of primers derived from the BAC end sequences, using glycerol stocks as templates. The findings reveal critical insights into the relationships and overlaps of these clones, providing valuable information for future genetic mapping and analytical studies.

ethan-floyd
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Analysis of Cross Amplification Overlaps in BAC Clones

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  1. 86B15 y 210 kb 91B12 t y 52O15 y t 94B05 y t 225 kb 90F15 t y 180 kb 86A03 y t 220 kb 80D12 y t 225 kb 49A10 t y 77J06 t y 77J06 y t 91A06 y t 65F21 x x 57A03 y t 80N03 t y 68N07 y Figure s2. The working map indicating the overlaps through cross amplification. Lines indicate the extension of selected BAC clones. Vertical arrows indicate the successful amplification of the primers from the BAC end sequence (arrow root end) using the BAC glycerol stock as template (point end)

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