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Cell Separation Using Magnetic Beads. MiniMACS ® Separator. MS Column. Magnet. MACS ® Technology. MACS Technology Used For Cell Separation Magnetic Activated Cells Sorting Yields Relatively High Purity Cell Populations 3 Components Involved MACS MicroBeads MACS Column MACS Separator.

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slide2

MiniMACS® Separator

MS Column

Magnet

macs technology
MACS® Technology
  • MACS Technology Used For Cell Separation
    • Magnetic Activated Cells Sorting
  • Yields Relatively High Purity Cell Populations
  • 3 Components Involved
    • MACS MicroBeads
    • MACS Column
    • MACS Separator
macs technology4
MACS® Technology
  • MACS MicroBeads
    • 50 nm in size
    • Biodegradable
    • Conjugated to monoclonal antibodies
  • MACS Column
    • Generates strong magnetic field
magnetic separation

Trapped Labeled Cells

High Gradient

Magnetic Field

Magnetic Separation
direct labeling
Direct Labeling
  • Advantages
    • Fast
    • Yields High Purity Cell Populations
  • Disadvantages
    • Binding of antibody to surface marker may activate cell
    • Some cell populations may require multiple markers to be identified
indirect labeling10
Indirect Labeling
  • Useful in cases with dim surface marker expression
  • Allows for amplification of signal
  • Secondary antibody recognizes
    • Biotin
    • FITC
    • Fc portion of primary antibody
  • Streptavidin instead of secondary antibody
    • Recognizes biotin on primary antibody
depletion strategy
Depletion Strategy
  • Unwanted Cells Are Magnetically Labelled And Depleted
  • Useful In Following Cases
    • Removal of Unwanted Cells
    • No specific antibody is available for target cell
    • Binding of antibody to target cell results in activations
protocol for isolation of cd4 cells17
Protocol For Isolation Of CD4+ Cells
  • 1. Prepare degased buffer