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MAKING NEW BODY PARTS

MAKING NEW BODY PARTS. BIOT 412, 2011 student. Making a New Bladder. Neurogenic bladder disorder Cystoplasty; a surgical treatment What we did??? Human bladder was engineered !. How???. Perform bladder biopsy; for urothelial and muscle cells. In vitro culturing and expansion of cells.

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MAKING NEW BODY PARTS

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  1. MAKING NEW BODY PARTS BIOT 412, 2011 student

  2. Making a New Bladder • Neurogenic bladder disorder • Cystoplasty; a surgical treatment • What we did??? • Human bladder was engineered!

  3. How??? • Perform bladder biopsy; for urothelial and muscle cells. • In vitro culturing and expansion of cells. • Seeding of cells on a biodegradable bladder-shaped scaffold. • New organ was ready after 8 weeks.

  4. Regenerated bladder

  5. Contd… • 5. Anastomosis • 6. Apply fibrin glue. • 7. Omental wrapping. Anastomosis

  6. Omentum layer

  7. Omental wrapping

  8. Results • After 46 months: • Improved function & capacity of ureter. • Renal function was normal through out the follow up. • No metabolic complication occured.

  9. Positives: • No immuno-suppression required! • Negatives: • No immediate Reconstruction of Vascular supply. • Instant Oxygen and nutrients supply is by diffusion. • Inflammation before re-angiogenesis.

  10. 2.Applying Regenerative Medicine toUpper Airways

  11. Making a New Trachea • End stage bronchio-malacia in a 30 year old woman, for example. • What we did??? • Ex- vivo bioengineering of trachea.

  12. How??? • Retrieving of deceased donor trachea. • Decellularization. • Obtaining epithelial cells from bronchial mucosa. • Isolation of stem cells from patient’s bone marrow. • Differentiation of SCs into chondrocytes. • 4 days maturation. • Human trachea was used as natural scaffold. • Seeding with autologous epithelial cells and chondrocytes. • Implantation of construct to replace the diseased one.

  13. Trachea scaffold

  14. Culturing the trachea

  15. Results • After 18 months: • Imaging showed normal architecture. • Patient was doing well. • Advantage! • Use of natural/ human scaffold.

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