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The Calvin Cycle

The Calvin Cycle. Lab 4 Photosynthesis. This lab will measure the rate of photosynthesis Under normal conditions an excited (mighty) electron will move through the ETC and reduce _______. Lab 4 Photosynthesis. This lab will measure the rate of photosynthesis

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The Calvin Cycle

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  1. The Calvin Cycle

  2. Lab 4 Photosynthesis • This lab will measure the rate of photosynthesis • Under normal conditions an excited (mighty) electron will move through the ETC and reduce _______.

  3. Lab 4 Photosynthesis • This lab will measure the rate of photosynthesis • Under normal conditions an excited (mighty) electron will move through the ETC and reduce NADP+. • What is going to happen in this lab?

  4. What happens as light hits the reaction center? DPIP

  5. The DPIP will be reduced, and it will go from blue to colorless DPIP How will we measure the rate of reduction of DPIP?

  6. Tips for Success • Be sure to add all materials to each cuvette, except the chloroplasts • Add chloroplasts to your blank cuvette and calibrate the Spec (colorimeter) • Once the machine has been calibrated, then add chloroplasts to one cuvette and immediately take your first reading.

  7. Use lens paper to keep cuvettes clean. • Mix each sample before you read them • Orient the cuvettes in the spec the same way each time you read them. • Keep all tubes a constant distance from the light source. • Remove the “dark” cuvette from the foil just before getting each reading.

  8. Predict Results:

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