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Identifying the Repressor and S toperator sequences for the A3 cluster phages

Identifying the Repressor and S toperator sequences for the A3 cluster phages. Ashley Holm, Laura Robey , Jacob Stoffel. Summary of results

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Identifying the Repressor and S toperator sequences for the A3 cluster phages

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  1. Identifying the Repressor and Stoperator sequences for the A3 cluster phages Ashley Holm, Laura Robey, Jacob Stoffel Summary of results From the tree we created using the DNA of the repressor gene, we feel that there should be a separation of the A3 subcluster. Using the data we collected from DNA Master, we determined that there should also be two different groups based on the stoperator sequences. Data The stoperator sequence results showed two distinct groups. Phantastic had only one hit but when looking at Misomonster (only 3 shown) there were 21 hits showing the distinct second group. All of the other phages were very similar to one of these two phages Abstract We set out to look at both the repressor and stoperator sequences in the A3 phages. We first looked at the repressor genes in the Mega program and created trees to show the relationships between the phages in the A3 subcluster. We then uploaded the DNA sequences of the phages into DNA Master and looked for the specific stoperator sequence within the genome. Overall, we found that there were two very distinct groups within the subcluster. Conclusions/Discussion Over all our data matches our hypothesis. We believe that there should be two groups from the subcluster A3. Introduction We originally suspected that there were at least two groups of phages within the A3 subcluster. In order to figure out if this hypothesis was true we looked at the repressor sequence and the stoperator sequence to confirm our suspicions. To complete this we used PhagesDB.org, Mega Software, and DNA Master. • References /Tools Used • Phagesdb.org • DNAMaster Program • CaseIt File-Mega Software Methods When working with the repressor genes we uploaded the sequences from phagesdb into the Mega Software. This process gave us trees that compared the genomes of the multiple phages in the A3 subcluster. We then used DNA Master to search for the stoperator sequence within the phages. From the phylogenic tree made from the repressor sequences we were able to see two distinct groups. The highlighted blue square is group one, matching our hypothesis. Acknowledgements Dr. Fred Bonilla Dr. Karen Klyzeck Dr. Kim Mogen

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