1 / 32

Bioremediation: The current and the future

Bioremediation: The current and the future. Graeme Paton Technical Director, Remedios Diarmuid O’Sullivan Senior Environmental Consultant. Bioremediation.

emily
Download Presentation

Bioremediation: The current and the future

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Bioremediation:The current and the future Graeme Paton Technical Director, Remedios Diarmuid O’Sullivan Senior Environmental Consultant

  2. Bioremediation • “ The act of stimulating the metabolism or co-metabolism of contaminants in the natural environment. Can be ex-situ, in-situ or involve natural attenuation” • 6 months - 3 years (ex-situ usually quicker)

  3. Endoenzyme Exoenzyme ?? Extracellular enzymes Exoenzyme Endoenzyme Dimers Oxidation Monomers CO2 Cell ½ O2 H2O Decomposition of biopolymers

  4. Tool Kit • 3 components • Induction • Constraints • Community

  5. Induction • Catabolic biosensors enable a prediction of the bioavailability of the target molecule in that matrix • Hence if the genes for degradation are in the matrix, degradation is very likely

  6. Tool Kit • 3 components • Induction • Constraints • Community

  7. Procedure for lux-based assay 100 % % of the control luminescence 10 % Increasing toxicity

  8. Tool Kit • 3 components • Induction • Constraints • Community

  9. MPN- Tetrazolium Paton et al 2003

  10. Site Location- Oil spill 1

  11. Tool Kit- at oil spill Toxicity Induction mpn TPH

  12. Kuwait Oil Lake- Oil spill 2 • During the first Gulf war (1990-1991) 50 million tonnes of soil was contaminated • Air-borne oily material contaminated one thousand square kilometres of soil.

  13. Systematic Grid

  14. Can we predict? Bioremediation (f) [TPH] (f) availability (f) degraders (f) constraints Induction x [TPH] mpn x (1-EC) BF=

  15. Prediction Once all of the data have been integrated an estimation of the likely case for degradation can be made Iroegbu et al., 2003

  16. TPH Week 0 Week 15

  17. Prediction v Actual

  18. Prediction • There is a close relationship between the predicted value and the actual hydrocarbon concentration. • This could define the end-point • Regular monitoring confirmed the progress of this work • This has been extended to over twenty trials to assess the value of the data

  19. Prediction Max specific deg rate mg TPH/ day Bioremediation Factor

  20. Prediction Max specific deg rate mg TPH/ day Bioremediation Factor

  21. Gaswork Site- 10,000 tonnes

  22. Bulk chemical- 2,500 tonnes

  23. Coupling Technologies

  24. The Big Issues On four occasions we have been required to seed/ augment a biological treatment process. This is a growing issue and a commercial opportunity.

  25. Pre-condition Nutrients Inoc. Source Substrate 1, 2, 3, 4 Remedios Soil Solution Bioaugmentation Agents

  26. Examples of Data

  27. What does this mean? Oil Technics- cfu= 108; mpn- 107 Supplier 1- cfu= 106; mpn- 104 Supplier 2- cfu= 106; mpn- 105 Supplier 3- cfu= 104; mpn- 103 Oil Technics and precondition- cfu= 109; mpn- 107 Supplier 1 and precondition- cfu= 108; mpn- 105 Supplier 2 and precondition- cfu= 107; mpn- 106 Supplier 3 and precondition- cfu= 105; mpn- 103

  28. Respiration Respiration Days

  29. Degradation TPH Days

  30. Prediction Max specific deg rate mg TPH/ day Bioremediation Factor

  31. Prediction Max specific deg rate mg TPH/ day Bioremediation Factor

  32. What does this mean • Augmentation is possible • Steps are required to refine • Immobilisation of wood is not ideal • Cost issues and value of application need to be considered • Need to focus and develop the skill-base

More Related