Effects of Alcohol on a 5 Day Old Chick Embryo’s Heart Rate Tami Christ Talia Katz Biology 240W Penn State Lehigh Valley
Background Information • Alcohol can have a good effect on the heart. • Moderate consumption can help protect against heart disease. • It can help raise HDL (good cholesterol) which helps to prevent plaque in arteries.
Background Information • Alcohol can have a negative effect on the heart. • Heavy consumption can lead to multiple diseases and heart complications. • Some disease and complicationsinclude: hypertension, alcoholic cardiomyopathy, congestive heart failure, and stroke.
Null Hypothesis • The null hypothesis of our experiment was that the alcohol would not have an effect on the heart.
Choosing the Concentrations • We chose the alcohol concentrations based on the amount of alcohol in everyday items. • 30%, which is the amount in mouthwash. • 10%, which is the amount in Nyquil. • 5%, which is the average amount in beer.
Making the Concentrations • In order to make the concentrations, started off with 80 proof alcohol (40% alcohol). • Then we used the 40% alcohol and gradually decreased the concentration using chick saline. • 30% = 3 parts alcohol and 1 part chick saline. • 10% = 2 parts 30% alcohol and 1 part chick saline. • 5% = 1 part 10% alcohol and 1 part chick saline.
Procedure • First, place tape on egg. • Then puncture a hole in egg with a scissors and withdraw some of the fluid from egg with syringe. • Cut out the window to reveal the embryo.
Procedure • Leave chick embryo in egg. • While in the egg, be sure to count the heart rate in vivo (bpm) with a stop watch. • Then explant the embryo from the egg with a tiny spoon and place in a dish filled with chick saline.
Procedure • Then move the embryo to a dish filled with CMRL. • Now count the heart rate in vitro (bpm). • Once the in vitro heart rate is taken, then explant the heart from the embryo’s body.
Procedure • Once the heart is explanted and the in vitro heart rate is counted, submerge the embryo’s heart in one of alcohol solutions. • Count the heart rate once placed into the dish of the alcohol solution.
Data Table Table 1
Future Experiments & Revisions • In future experiments, 1 change should be made. • The 3 concentrations of alcohol would need to be change. • The concentrations were way too high and stopped the heart almost immediately. • Changing the concentrations to ideal blood alcohol levels ( 0.08% < x < 0.08%).
Summary • We really did not get a clear answer to our hypotheses. • I think with a future experiments and the revisions made we would have a clearer answer to how alcohol and its concentration affect the heart. • With the information gained from the experiment, we have a better idea for future experiments.
References • “Alcohol and Heart Disease.” Women’s Heart Foundation. 2007. <http://www.womensheart.org/content/HeartDisease/alcohol_and_heart_disease.asp>. • McLaughlin, Jacqueline, McCain, Elizabeth. “Student Outline.” 1999. <http://www2.lv.psu.edu/jxm57/chicklab/outline.html>. • Norr, Serena. “How Does Alcohol Affect the Heart?” 2009. <http://i.ehow.com/images/a04/m8/58/alcohol-affect-heart-800X800.jpg>.