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Microarray data: overview. mRNA. cDNA. Normal cell. RT. cDNA. mRNA. Tumour cell. Microarray data: overview. cDNA samples are mixed together and hybridize to complementary DNA on the microarray a laser scanner measures the fluorescence of each spot. Superimposed

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microarray data overview
Microarray data: overview

mRNA

cDNA

Normal cell

RT

cDNA

mRNA

Tumour cell

microarray data overview1
Microarray data: overview
  • cDNA samples are mixed together and hybridize to complementary DNA on the microarray
  • a laser scanner measures the fluorescence of each spot. Superimposed
  • fluorescence intensity is related to amount of cDNA in sample = mRNA = gene
microarray data overview2
Microarray data: overview
  • Data = thousands of different coloured dots
  • Colour intensity = number
  • EXAMPLE:
  • Four genes
  • Arbitrary light units
  • Ratio of red to green (red/green)
microarray data analysis
Microarray data: analysis
  • Thousands of genes + 10s, 100s of samples = DATA
  • Need to use specialized software
  • Two options: same destination, different style

* Free software (R/Bioconductor) * Commercial software (Partek/Genespring

£££ / $$$

microarray data partek
Microarray data: Partek

Gene analysis

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Exploratory

analysis

Gene lists

Clustering

Biological pathways

Add sample

information

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