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Eukaryotic Translation. mRNA tRNA rRNA. mRNA. -monocistronic -1,000-2,000 bases long -methylated at 5’cap -100-200 bases of poly A at 3’end -nontranslated 5’ reader < 100 bases -nontranslated 3’ tailer ~ 1,000 bases - AUG as the INITIATOR -UAA,UAG,&UGA as TERMINATOR.

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Eukaryotic Translation


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Presentation Transcript
slide4

mRNA

-monocistronic

-1,000-2,000 bases long

-methylated at 5’cap

-100-200 bases of poly A at 3’end

-nontranslated 5’ reader < 100 bases

-nontranslated 3’ tailer ~ 1,000 bases

- AUG as the INITIATOR

-UAA,UAG,&UGA as TERMINATOR

slide7

Life Cycle of Eukaryotic mRNA

-removal of Poly(A) need

for decapping enzyme

-Poly(A)-binding protein,

PABP on Poly(A) prevents the

decapping enzyme from

binding to the 5’end

slide8

AU-rich sequence,

called ARE

~50 bases in 3’ tailer

-AUUUA, more repeated

-trigger destabilization

slide9

TRE sequence

- contain strm-loop

structures for

protein binding

-Protein inhibit

destabilizing

slide10

tRNA

Met-tRNAifor initiation

Met-tRNAmforelongation

- No formylated Methionine

-has an unusual tertiary structure,

modofied by phosphorylation of

the 2’ribose position

slide12

The right context for Recognization

-AUG

-the most important seq.

GCCAGCCAUGG, purine

three bases before AUG and G

immediately following it.

CAP; for a stable complex

to be formed at initiation codon

slide13

Eukaryotic Initiation

*form ternary complex

*bind free 40S

*attach the 5’ end

Ternary complex;

-Met-tRNAi

-eIF2

-GTP

slide14

Initiation Factors

*bind 5’ cap

*unwind mRNA

*bind subunit initiation

complex

*support 60S joining

slide15

Elongation Factors:

EF-Tu, load aminoacyl tRNA

EF-Ts,

GDP to GTP

slide16

Translocation:

Peptidyl transferase,

synthesis peptide

slide17

EF-Tu

EF-G