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The DNA Resources Core 518 Light Hall. Manager: Cara Sutcliffe 6-2744 [email protected] Faculty Director: Doug Mortlock 6-1671 [email protected] You’re collecting patient samples …Now what?. DNA extraction from blood or buccal swabs Storage of extracted DNA

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The dna resources core 518 light hall l.jpg
The DNA Resources Core518 Light Hall


You re collecting patient samples now what l.jpg
You’re collecting patient samples …Now what?

  • DNA extraction from blood or buccal swabs

  • Storage of extracted DNA

  • Secure, but practical database system for managing samples

  • DNA retrieval at a later time, for genotyping samples of interest

  • SNP genotyping service appropriate to your scale and needs

  • Compliant with HIPAA


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They are still your samples!

  • Control of samples belongs to the investigator

  • Password-protected, Web-based database

  • Non-authorized personnel cannot access sample information or place requisitions for DNA aliquots

  • “Outsourcing” extraction, storage and organization of DNA samples.


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DNA extraction from blood

  • Gentra Puregene reagent system

  • ~350 µg DNA from 10 ml blood

  • Autopure machine

  • Bar coded


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DNA archiving

  • DNA tubes: ID #, bar coded

  • Puregene reagent system:

    Purified DNA is stored dissolved in TE at 4˚. Stable for >10 years


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DNA extraction: standard service

  • DNA Extraction from Whole Blood

    For 10mls (or 20mls) of blood, services include:

    • logging in and maintenance of sample information in our database,

    • DNA extraction,

    • archiving backup blood spot cards and frozen blood aliquots,

    • DNA quantitation by fluorometry,

    • one DNA requisition,

    • storage of extracted DNA for 5 years.


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Additional services

  • Lymphoblast immortalization & cell line banking

  • Whole-genome amplification for depleted DNAs

  • Real-time PCR


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Genotyping services

  • ABI 7900HT machine

    (Dan Roden/Pharmacogenetics of Arrythmia Therapy)

  • TaqMan SNP assays

    Typically >99% accurate

  • Assays-on-demand:

    Pre-validated SNP assays by ABI

  • Assays-by-design:

    For “custom” SNPs; need to be validated before use


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Genotyping services

  • ABI 7900HT machine

    (Dan Roden/Pharmacogenetics of Arrythmia Therapy)

  • TaqMan SNP assays

    Typically >99% accurate

  • Assays-on-demand:

    Pre-validated SNP assays by ABI

  • Assays-by-design:

    For “custom” SNPs; need to be validated before use


Genotyping services10 l.jpg
Genotyping services

• Service models using the ABI 7900HT genotyping platform:

1. “Full service”: Customer selects Assays-on-demand; core staff set up TaqMan PCR assays in 384 well plates, and scan plates on 7900HT to collect genotypes.

($400 setup + $0.80/genotype)

2. As 1, and core staff assist with designing custom Assays-by-design (inquire)

3. Customer obtains Assays-by-design; core staff perform PCR and scan plates.

($95 setup + $0.80/genotype)

4. Customer is responsible for setting up PCR and scanning plates ($10/plate).


The dna resources core 518 light hall11 l.jpg
The DNA Resources Core518 Light Hall


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