slide1
Download
Skip this Video
Download Presentation
Absorptive cells Enterocyte : nutrient absorption

Loading in 2 Seconds...

play fullscreen
1 / 18

Absorptive cells Enterocyte : nutrient absorption - PowerPoint PPT Presentation


  • 188 Views
  • Uploaded on

Cells proposed to be derived from ISCs at the crypt base. Other Tuft cells M-cells. Absorptive cells Enterocyte : nutrient absorption. Secretory cells Goblet cell: mucus secretion Paneth cells: antimicrobial product secretion Enteroendocrine cells: hormone secretion.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Absorptive cells Enterocyte : nutrient absorption' - elga


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1
Cells proposed to be derived from ISCs at the crypt base

Other

Tuft cells

M-cells

Absorptive cells

Enterocyte: nutrient absorption

Secretory cells

Goblet cell: mucus secretion

Paneth cells: antimicrobial product secretion

Enteroendocrine cells: hormone secretion

slide2
Colon similar to small intestine:

Similar cell types are derived from ISCs at the crypt base

slide4
ISCs – Crypt base columnar (CBC) cells or +4 cells?

+4 cells were proliferating, label-retaining cells: proposed to possess an immortal DNA strand maintained by asymmetric cell division

CBC cells were traced to all the lineages and formed crypts when cultured ex vivo

slide5
Lgr5 expression restricted to CBC cells

Lgr5 expression

Marks CBCs

Barker N. et al, Nature 449:1003, 2007

slide7
Use of an Lgr5-GFP-Cre transgene to mark Lgr5-expressing cells and their descendents

CBC stem cells mapping below +4

1 5 60 days following Cre activation

slide8
Single Lgr5+ cells generate crypt–villus structures.

T Sato et al. Nature000, 1-4 (2009) doi:10.1038/nature07935

slide9
Composition of single stem cell-derived organoids.

T Sato et al. Nature000, 1-4 (2009) doi:10.1038/nature07935

slide10
Fun facts:

Lgr5 ISCs do not undergo asymmetric division – they divide symmetrically, producing either 2 TA cells or 2 ISCs.

No evidence of an immortal DNA strand in Lgr5 ISCs obtained

Barker N. et al, Nature 449:1003, 2007

slide11
An Lgr5-Diptheria toxin receptor fusion gene can trigger the elimination of Lgr5-expressing cells

Tian et al. Nature 2011

slide14
Lineage tracing shows Bmi1 cells contribute cells to the crypt and this contribution increases when Lgr5 cells are eliminated
ad