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Flag-FOXO3a. DAPI. Merged. Untreated. WT MEFs. Doxorubicin (16h ). Untreated. p38α -/- MEFs. Doxorubicin (16h). Supplementary Figure S7. The transfected Flag-FOXO3a accumulates in the nuclei of the wild-type but not p38α-deficent MEFs following doxorubicin treatment

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slide1

Flag-FOXO3a

DAPI

Merged

Untreated

WT MEFs

Doxorubicin

(16h)

Untreated

p38α-/-MEFs

Doxorubicin

(16h)

Supplementary Figure S7. The transfected Flag-FOXO3a accumulates in the nuclei of the wild-type but not p38α-deficent MEFs following doxorubicin treatment

Wild-type and p38α-deficent MEFs were seeded into slide chamber, transfected with the pCMV-Flag-FOXO3a (WT) and incubated with media in the absence or presence of doxorubicin for 16 h. Immunofluorescent staining was then performed using the Flag antibodies and DAPI. All images shown are typical results obtained from at least 10 different fields.