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Experiment 1. Experiment 2. Experiment 3. Laforin -/- x Amylase. Malin -/- x Amylase. TAT-Amylase. Characterization of KO mouse. Breeding set-up. Experimental set-up. 0M. PCR, Molecular cloning. Genotyping, Animal caring. Genotyping, Animal caring. 3M.

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12M

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  1. Experiment 1 Experiment 2 Experiment 3 Laforin-/- x Amylase Malin-/- x Amylase TAT-Amylase Characterization of KO mouse Breeding set-up Experimental set-up 0M PCR, Molecular cloning Genotyping, Animal caring Genotyping, Animal caring 3M Establishing Malin -/- mouse line for breeding Obtaining laforin -/- in Amylase background Obtaining Malin-/- in Amylase background TAT-Amylase construct finished Extensive trials for TAT-Amylase purification 6M TAT-Amylase purification finished 9M Breeding with Amylase mouse Animal caring Animal caring Test in cell lines Mouse grown >6M for LB developing, and fed with Dox. TAT-Amylase test in cell lines, ready for testing in mice 12M Please note that the breeding for Malin-/- and Amylase mice (Experiment 2) is approximately 6 months later than that of Laforin-/- and Amylase mice (Experiment 1).

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