effect of bile salts on pancreatic lipase activity n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Effect of Bile Salts on Pancreatic Lipase Activity PowerPoint Presentation
Download Presentation
Effect of Bile Salts on Pancreatic Lipase Activity

Loading in 2 Seconds...

play fullscreen
1 / 8

Effect of Bile Salts on Pancreatic Lipase Activity - PowerPoint PPT Presentation


  • 250 Views
  • Uploaded on

Effect of Bile Salts on Pancreatic Lipase Activity. Introduction. Pancreatic lipase , is secreted from the pancreas, that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Effect of Bile Salts on Pancreatic Lipase Activity' - dore


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide2

Introduction

Pancreatic lipase, is secreted from the pancreas, that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids.

slide3

Bile consists of watery mixture of organic and inorganic compounds.

Bile can either pass directly from the liver where it is synthesized into the duodenum through the common bile duct where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat,

slide4

which allows the lipase to break apart the fat more effectively,

or bile stored in the gallbladder when not immediately needed for digestion

slide5

Bile salts are important in the digestion of fats because they lower the surface tension of the fatty materials which enables them to be emulsified in an aqueous medium. As emulsifiers, they greatly increase the surface area of the fatty substrate and hasten the hydrolysis by increased enzyme catalysis

Surface tension is a property of the surface of a liquid that allows it to resist an external force

slide6

Method

B

A

0.5ml Vegetable oil

1.0 ml H2O

2ml Pancreatin solution

0.5ml Vegetable oil

1.0 ml Sodium cholate

2ml Pancreatin solution

Mix and cover the flasks,

then incubate at 45C for 90 min

slide7

Shake by rotation each of flasks at 15min intervals

Then add 8 drops of phenolphthalein indicator to both flasks and titrate against 0.05 N sodium hydroxide to a distinctly permanent pink (30 sec at least)end point.

Record the titration volumes and calculate the meg. y free fatty acids produced with and without the presence of bile salts.

slide8

Calculation

C1 X V1 = C2 X V2

Blank:

1- 0.01 X V1(NaOH) = C2 X 0.5

Sample:

2- 0.01 X V1 = C2 X 0.5

1 -2 = Net free fatty acid in 0.5ml of fat