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Thiamin, Riboflavin, and Niacin

Thiamin, Riboflavin, and Niacin. By: Kaitlin Deason and Confidential Group Members. Objectives:. Brief history and fun facts of thiamin, riboflavin, niacin Overview of absorption, digestion, and transportation Overview of RDAs, sources, deficiencies, toxicities, and assessment tests

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Thiamin, Riboflavin, and Niacin

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  1. Thiamin, Riboflavin, and Niacin By: Kaitlin Deason and Confidential Group Members

  2. Objectives: • Brief history and fun facts of thiamin, riboflavin, niacin • Overview of absorption, digestion, and transportation • Overview of RDAs, sources, deficiencies, toxicities, and assessment tests • Overview of metabolism

  3. Thiamin Vitamin B1

  4. History • 1880s Dr Takaki: relationship between “the nitrogenous substances …in the food” and the disease beriberi (I can’t –I can’t) in Japanese Navy • 1890 Diet to prevent beriberi was written into law • 1886 Dr. Christian named beriberi as polyneuritis gallinarum • “anti-polyneuritis factor” could be extracted from rice hulls with water and ethanol

  5. History con’t. • 1911 Dr. Funk crystallized an amine substance from rice bran • 1926 Dr. Jansen and Dr. Donath crystallized vitamin B1 from rice bran namedantineuriticvitamin; however, they missed the sulfur atom and their formula was incorrect • 1936 Williams published the correct formula • Thiamine as reflection of amine nature of vitamin

  6. Thiamin: Absorption Transport Storage • Water soluble vitamin • Absorption in the jejunum • Passive diffusion if thiamin intake is high • Active diffusion Sodium Dependent if thiamin intake is low • Ethanol ingestion interferes with active transport of thiamin • In the blood bound to albumin • Storage: 30 mg • ~90 % within blood cells • Small amount in the liver, skeletal muscles, brain, heart, kidney

  7. Thiamin: Main Active FormsThiamin Di- or Pyrophosphate (TDP/TPP)

  8. Thiamin: Main Active Forms Thiamine triphosphate (TTP) Thiamine di-phosphate + ATP  Thiamine triphosphate (TTP)+ ADP

  9. Metabolism Thiamin: Energy Transformation TDP in Enzyme Systems Oxidative Decarboxylation of • Pyruvate • -ketoglutarate • Three branched chain amino acids: isoleucine, leucine and valine

  10. Physiological & Biochemical functions • Noncoenzyme: Membrane and nerve conduction • Coenzyme: • Energy transformation • Synthesis of pentoses and NADPH

  11. Recommended Daily AmountsRDA Men: 1.2 mg/day Women: 1.1 mg/day Pregnant: 1.4 mg/day Lactating : 1.5 mg/day

  12. Sources of thiamin • Excellent sources: • Pork and Sunflower seeds • Good sources: • Enriched and fortified or whole grains: (bread, ready-to-eat cereals)

  13. Funny fact • If you can’t get enough of sushi you might want to think twice. Raw fish contains thiaminase – an enzyme that deactivates thiamin. Cooking fish makes the enzyme inactive.

  14. Thiamin Deficiency: Groups at Risk • Biological half-life of thiamin in the body is about 15 days, deficiency symptoms can be seen in people on a thiamin deficiency diet as little as 18 days. • Groups with a greater risk: • individuals with kidney diseases on dialysis • Malabsortion syndrome or genetic metabolic disorders • Pregnant women with more then one fetus • Seniors • Chronic dieters • Elite athletes • Alcoholics

  15. Thiamin Deficiency • Beriberi –true deficiency is not common in USA • Dry beriberi from low thiamin intake in older adults • Wet beriberi with cardiovascular system involvement • Acute beriberi in infants • Failure to oxidize -keto acids from leucine, isoleucine and valine causes accumulation in blood the branched –chain acids • Findings are characteristic of Maple Syrup Urine Disease (MSUD)

  16. Thiamin Deficiency Symptoms • Associated with alcoholism • Wernicke-Korsakoff Syndrome: muscle wasting and encephlopathy • Mental confusion • Speech difficulties • Nystagmus • Diarrhea • Edema • Fatigue • Weight loss • Burning pain in the extremities • Ataxia • Coma • Heart failure

  17. Toxicity Symptoms • Oral intake of 500 mg/day for 1 month • Headache • Convulsion • Cardiac arrhythmia • Anaphylactic shock • No tolerable upper intake level

  18. Assessment Thiamin • Measurement of erythrocyte transketolase activity ( an increase in transcetolase activity >25% indicates thiamin deficiency • Measurement of urinary thiamin excretion • Clinical response to administered thiamin (symptoms improve after the person is given thiamin supplements)

  19. Thiamin: Disease implications Benfotiamin- lipid-soluble thiamin derivative can activate pentose phosphate transketolase to prevent experimental retinopathy Hammer, H-P, Du, X., Edelstein, D (2003) Benfotiamine Blocks Three Major Pathways of Hyperglycemic Damage and prevents Experimental Diabetic Retinopathy. Nature Medicine, 9,3,294-299  Case study: 5-week girl was hospitalized due heart failure. The infant was diagnosed with dilated cardiomyopathy. Parents refused the heart transplantation and treatment with thiamine hydrochloride was started. 48 hours later the patient condition was improved, suggestion that her condition was due to defect of thiamin intake. Conclusion: All patient with early dilated cardiomyopathy should have their thiamin plasma measured. Rocco, M.D., Patrini, C., Rimini, A. (1997) A 6-month-old Girl with Cardiomiopathy Who Nearly Died. Lancet, 349, 616

  20. Riboflavin Vitamin B2

  21. Description of Riboflavin • Water Souble Vitamin • Riboflavin = Flavin + Ribitol • Flavin means yellow in Latin • Ribitol is a alcohol sugar • Yellow fluorescent characteristic of Riboflavin comes from Flavin • Greatest concentrations of B2 found in liver, kidneys, and heart Ribitol Flavin http://themedicalbiochemistrypage.org/images/riboflavin.jpg

  22. History of Riboflavin • 1933 Riboflavin was discovered by Kuhn, Szent, Wagner • In the US, originally known as vitamin G • Riboflavin’s unique fluorescent orange-yellow color help researchers identify B2 http://sandwalk.blogspot.com/2007/09/nobel-laureate-richard-kuhn.html

  23. Main Coenzymes • FMN - Flavin Mononucleotide • FAD - Flavin Adenine Dinucleotide • Most commonly found in foods • In the intestinal lumen the coenzymes are converted into riboflavin FAD FAD pyrophosphatase FMN phosphatase FMN Riboflavin

  24. Physiological and Biochemical Functions of Riboflavin • Main Function - Electron Hydrogen Transfer Reactions • Oxidative Decarboxylation of pyruvate • SuccinateDehydrogenase • Fatty Acid Oxidation • SphinganaineOxidase • XathineOxidase • AldehydeOxidase • Pyridoxine phosphate oxidase • Active form of folate • Synthesis of niacin from tryptophan • Choline Catabolism • Thioredoxinreductase • Monoamine oxidase • Oxidized form of glutathoine

  25. Metabolism of Riboflavin • Riboflavin most commonly found bonded to protein in foods. • Prior to absorption, riboflavin must be freed of the protein • Divalent metals such as Copper, Zinc, Iron inhibit the absorption of riboflavin • Alcohol – impairs Riboflavin digestion and absorption • ~ 95% Riboflavin is absorbed from foods up to 25 mg • ~7% of FAD is covalently bound to AAs; Histidine or Cysteine, can’t function in the body and remains bound • Excreted in the urine

  26. Absorption of Riboflavin Mucosal cells: Riboflavin FMN Serosal surface: FMN is dephophorylated to Riboflavin B2 is transported to the liver Converted to FMN or other coenzyme by flavokinase FAD is most predominant flavoenzyme in tissues Flavokinase ATP ADP

  27. Transportation of Riboflavin Systemic plasma Most flavins are found as riboflavin Riboflavin, FMN, and FAD are transported in the plasma by a variety of proteins Albumin, fibrinogen, and globulins Albumin is the primary transport protein Free riboflavin uses carrier mediated process to traverse most cell membranes In the brain riboflavin uses a high affinity transport system for B2 and FAD

  28. Deficiency of Riboflavin • Ariboflavinosis • Cheilosis – lesions on outside of lips • Angular Stomatitis – Corners of mouth • Glossitis – Inflammation of tongue • Hyperemia – Redness or bleeding in oral cavity • Edema – swollen mouth/ oral cavity • Seborrheic Dermatitis – inflammatory skin condition • Anemia • Nueropathy- peripheral nerve dysfuction

  29. Populations with greatest risk of deficiency • Congentialheart disease • Some Cancers • Excess alcohol intake • Thyroid disease • Diabetes Mellitus, trauma, stress • Women who take oral contraceptives

  30. Sources of riboflavin • Excellent Sources – animal origin products • Beef Liver, Sausage, Steak, Mushrooms, Ricota Cheese, Nonfat Milk, Oysters • Significant Sources – Eggs, meat, legumes • Fairly Good Sources – Green Vegetables • Minor Sources – Fruit and Cereal grains

  31. Forms of Riboflavin in Foods • FMN and FAD • Most common • Free or protein bound • milk, eggs, enriched breads and cereals • Phosphorous bound

  32. RDA of Riboflavin • Men – 1.3 mg/day • Women – 1.1 mg/day • Pregnant – 1.4 mg/day • Lactating – 1.6 mg/day

  33. Toxicity Levels of Riboflavin • Level has yet to be determined • Fun Fact - 400 mg of Riboflavin – is an effective treatment dose for migraine headaches without any side effects

  34. Assessment of Riboflavin • Erythrocyte glutathione reductase • Good measurement because requires FAD for a coenzyme • If reaction is limited than Riboflavin intake is low

  35. Riboflavin disease Implications • Riboflavin increase lowers homocysteine reducing the risk of coronary atherosclerosis • Riboflavin and folate work together to reduce plasmatHcy (total homocysteine) Moat, S., Pauline A. L., Ashfield-Watt, Powers, H. J., NewcombeR.G, and McDowell, I. (2003). Effect of Riboflavin Status on the Homocysteine-lowering Effect of Folate in Relation to the MTHFR (C677T) Genotype. Clinical Chemistry. 2003;49:295-302 • Riboflavin can increase the amount of antioxidants in a breast cancer patient, increasing DNA repair • Supplemented with 100 mg co-enzyme Q10, 10 mg riboflavin and 50 mg niacin (CoRN), one dosage per day along with 10 mg tamoxifen twice per day. • Premkumar,V. G., Yuvaraj, S., Shanthi P., and Sachdanandam, P . (2008). Co-enzyme Q10, riboflavin and niacin supplementation on alteration of DNA repair enzyme and DNA methylation in breast cancer patients undergoing tamoxifen therapy. British Journal of Nutrition 100: 1179-1182

  36. Niacin Vitamin B3

  37. History of Niacin • Niacin was discovered because of its deficiency pellagra • Documentation of pellagra dates back to the 1760’s in Spain and Italy • Joseph Goldberger was the first to come up with a scientific reason to explain pellagra • He discovered that pellagra could be cured by milk and concluded that it was not an infectious disease • Continuing the work of Joseph Goldberger, Conrad Elvehjem was able to isolate and identify niacin. • Fun fact: Originally, referred to as only nicotinamide, it was renamed to niacin because it was thought that nicotinamide too closely resembled nicotine and the didn’t want people getting confused and thinking they were harming themselves or that cigarettes contained vitamins.

  38. Niacin is the general term to classify both nicotinic acid and nicotinamide Suave, A. A. (2007). NAD+ and Vitamin B3: From metabolism to therapies. The Journal of Pharmacology and Experimental Therapeutics, 324(3), 883-893.

  39. Absorption • Most absorption of niacin occurs in the small intestine. • Absorption/transportation occurs in one of two ways: • Passive diffusion- this happens when it is at high concentrations (ex. Pharmacological doses) • Facilitated diffusion- This is a sodium dependent reaction that occurs when niacin is in lower concentrations

  40. Transportation • Niacin is transported through the blood stream and then is able to move across cell membranes by simple diffusion • The exception is when nicotinic acid is being transported into the kidney tubules or the RBC’s. This requires a carrier. • However, this is not very often because in the blood plasma, niacin is most commonly in the form of nicotinamide • Niacin is used by all tissues so it is transported throughout the body

  41. Importance of Niacin • Nicotinamide is the primary precursor for NAD and NADP • Approximately 200 enzymes require NAD or NADP • NADNADH: main role is to transport electrons through the ETC, but also acts as a co-enzyme for: • Glycolysis • β-oxidation of fatty acids • Oxidative decarboxylation of pyruvate • Oxidation of acetyl CoA via Krebs cycle • Oxidation of ethanol

  42. Importance of Niacin cont. • NADPNADPH: main role is as a reducing agent in the hexosemonophosphate shunt but also also acts as a co-enzyme for: • Fatty acid synthesis • Cholesterol and steroid synthesis • Oxidation of glutamate • Synthesis of deoxyribonucleotides • Regeneration of glutathionine, vit. C, and thioredoxin • Folate metabolism

  43. Mechanism of action • NAD+ and NADP act as electron acceptors (and donors) Boyer, R. (2002). Concepts in biochemistry. Canada: John Wiley and Sons. Fig. 16.7

  44. Synthesis of Niacin • Our body can synthesize NAD from the amino acid tryptophan in the liver. • This requires other vitamins and minerals. • Despite this, we still require niacin from dietary sources. WHY? • This only happens when we have adequate amounts of tryptophan, AND it only occurs at a rate of 60:1. This ends up being about 3% of tryptophan being used to synthesize NAD

  45. RDA for Niacin • The RDA is expressed in niacin equivalents (NE) • For men: 16 mg (NE)/day • For Women: 14 mg (NE)/day • During pregnancy and lactation this increases to 18 mg (NE) and 17mg (NE)/day • To determine NE we assume the 60:1 mg tryptophan to niacin ratio • Approximately 1% of each gram of protein is tryptophan

  46. Sources of Niacin • Foods high in protein such as, fish*, chicken*, beef, and pork • Enriched/fortified breads and cereals • Legumes • Small amounts from dairy products and green vegetables • *Excellent sources are chicken breast and canned tuna http://www.nlm.nih.gov/medlineplus/mobileimages/ency/fullsize/18104_xlfs.png

  47. Calculating NE • Determine RDA for protein. • 0.8g/kg body wt. So, for someone who weighs 61 kg they need 49g of protein • Anything above this (leftover protein) will be used to convert to niacin. So lets say this person eats 79g protein • Divide leftover protein by 100 to determine grams of tryptophan and then x1000 to get mg • Finally divide by 60 to determine niacin mg synthesized • 79g-49g= 30g ; 30g÷100=0.3 g tryptophan ; 0.3x1000= 300mg tryptophan ; 300mg tryptophan÷60= 5mg niacin

  48. Pellagra: niacin deficiency • Characterized by the 4 D’s: • Diarrhea • Dermatitis • Dementia • Death Fred, H. L., & Van Dijk, H .A. (2007). Images of memorable cases: 50 years at the bedside. Houston: Long Tail Press/Rice University Press.

  49. Pellagra cont. • Niacin can be covalently bound to proteins (niacinogen) or carbohydrates (niacytin) • The covalent bond is not sensitive to HCl in the stomach and therefore niacin is not released for absorption • Niacin is not absorbed and deficiency occurs • Niacinogen and niacytin are most common in corn which was a major source of food during the depression • Now we know how to solve the problem

  50. Niacin deficiency • Besides pellagra, deficiency or diminished niacin status can also occur Populations at risk: • Those taking certain medications (Ex. Antituberculosis drug isoniazid) • Malabsorptive disorders- chronic diarrhea, inflammatory bowel disease, some cancers… • Those with Hartnup disease- impairs tryptophan absorption decreasing synthesis to niacin • Alcoholics

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