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Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Environment. Controlling Microbial Growth. Terminology of Microbial Control Sterilization Aseptic Disinfection Degerming Sanitation Pasteurization Stasis/static Cide/cidal. Controlling Microbial Growth. Physical Methods of Microbial Control Heat

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Controlling Microbial Growth in the Environment

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  1. Controlling Microbial Growth in the Environment

  2. Controlling Microbial Growth • Terminology of Microbial Control • Sterilization • Aseptic • Disinfection • Degerming • Sanitation • Pasteurization • Stasis/static • Cide/cidal

  3. Controlling Microbial Growth • Physical Methods of Microbial Control • Heat • Moist Heat • Boiling • Autoclaving • Pasteurization • Dry Heat • Refrigeration & Freezing

  4. Controlling Microbial Growth Contd- • Desiccation • Lyophilization • Filtration • Osmotic Pressure • Radiation

  5. Controlling Microbial Growth Contd- • Chemical Methods of Microbial Growth • Phenols and Phenolics • Denatures • Alcohols • Denatures • Halogens • Iodine used as antiseptics • Chlorine used in drinking water and as disinfecting bleach • Bromide used as a disinfectant in hot tubs because it evaporates more slowly than chlorine at high temperatures.

  6. Controlling Microbial Growth Contd- • Chemical Methods Contd- • Oxidizing Agents • Peroxides and Ozone • Reactive oxygen that kills microbes by oxidizing their enzymes and preventing their metabolism • Surfactants • Soaps and Detergents reduce the surface tension of solvents like water and disrupt cell membranes • Heavy Metals • Zn, arsenic, Hg, Cu, Ag,- alter 3-d shape of proteins

  7. Controlling Microbial Growth Contd- • Chemical Methods Contd- • Aldehydes • Compounds containing CHO group. Crosslink amino, COOH, OH Groups in proteins to denature them • Use to disinfect medical and dental equipment • Gaseous Agents • Used for sterilizing plastic labware, mattresses, pillows and things that cannot be easily sterilized by heat, chemicals or irradiation. Examples include ethylene dioxide and propylene oxide.

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