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Controlling Microbial Growth • Terminology of Microbial Control • Sterilization • Aseptic • Disinfection • Degerming • Sanitation • Pasteurization • Stasis/static • Cide/cidal
Controlling Microbial Growth • Physical Methods of Microbial Control • Heat • Moist Heat • Boiling • Autoclaving • Pasteurization • Dry Heat • Refrigeration & Freezing
Controlling Microbial Growth Contd- • Desiccation • Lyophilization • Filtration • Osmotic Pressure • Radiation
Controlling Microbial Growth Contd- • Chemical Methods of Microbial Growth • Phenols and Phenolics • Denatures • Alcohols • Denatures • Halogens • Iodine used as antiseptics • Chlorine used in drinking water and as disinfecting bleach • Bromide used as a disinfectant in hot tubs because it evaporates more slowly than chlorine at high temperatures.
Controlling Microbial Growth Contd- • Chemical Methods Contd- • Oxidizing Agents • Peroxides and Ozone • Reactive oxygen that kills microbes by oxidizing their enzymes and preventing their metabolism • Surfactants • Soaps and Detergents reduce the surface tension of solvents like water and disrupt cell membranes • Heavy Metals • Zn, arsenic, Hg, Cu, Ag,- alter 3-d shape of proteins
Controlling Microbial Growth Contd- • Chemical Methods Contd- • Aldehydes • Compounds containing CHO group. Crosslink amino, COOH, OH Groups in proteins to denature them • Use to disinfect medical and dental equipment • Gaseous Agents • Used for sterilizing plastic labware, mattresses, pillows and things that cannot be easily sterilized by heat, chemicals or irradiation. Examples include ethylene dioxide and propylene oxide.