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Effect of Heme Oxygenase-1 on the smooth muscle cells growth

Effect of Heme Oxygenase-1 on the smooth muscle cells growth. the biological function of heme oxygenase. +. Heme oxygenase. heme. CO. Fe2+. Biliverdin. Bilirubin. three isoforms of heme oxygenase (HO). HO-1 : an inducible isoform

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Effect of Heme Oxygenase-1 on the smooth muscle cells growth

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  1. Effect of Heme Oxygenase-1 on the smooth muscle cells growth

  2. the biological function of heme oxygenase

  3. + Heme oxygenase heme CO Fe2+ Biliverdin Bilirubin

  4. three isoforms of heme oxygenase (HO) HO-1 : an inducible isoform HO-2: a constitutive isoform HO-3: closely resembles HO-2

  5. IL-10 NO 15-PGJ2 UV radition Heat shock proteins hypoxia

  6. Protection effect of HO-1 by many mechnisms Anti-inflammation Anti-apoptotisis Anti-proliferation

  7. the effect of HO-1 on the smooth muscle cells growth

  8. Hmox1–/– Hmox1+/+ HO-1 gene deletion (Hmox1-/-) results in enhanced cell growth

  9. TransferAdHO-1 (adenovirus containing the rat HO-1 gene) to SMCs HO-1 AdHO-1 + SMC

  10. Infection of SMCs with AdHO-1 inhibit the proliferative response to serum in a concentration-dependent manner 2% serum 5% serum

  11. Mechanism Ⅰ HO-1 blocks the proliferation of vascular SMCs by stimulating programmed cell death caspase-3 activity DNA laddering

  12. DNA laddering investigate the effect of CO and Fe on cell apoptosis

  13. the effect of bilirubin on cell apoptosis Bilirubin Production Administration of biliverdin and bilirubin

  14. HO-1 Apoptosis bilirubin CO Fe

  15. Mechanism Ⅱ [3H] c.p.m. (%control) AdHO-1: AdHO-1 AdHO-1 AdHO-1 AdHO-1 AdHO-1 Inhibitor: - +ZnPPIX +L-NAME +ODQ +Rp8BrcGMP Target: - (HO-1) (NOS) (sGC) (cGMP)

  16. Control Ad-ΔE1 AdHO-1 Fluorescence intensity Cell number

  17. Summary

  18. Thanks!

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