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Lab Midterm Jeopardy

Test your knowledge and prepare for your lab midterm with this jeopardy-style game. Answer questions on lab safety, techniques, staining methods, and more. Play with a friend or challenge yourself alone!

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Lab Midterm Jeopardy

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  1. Lab Midterm Jeopardy Play with a friend or alone! Paulos Menos II

  2. Some quick things • This doesn’t cover everything. • The difficulty distribution of the questions is most likely not equal, I’m not perfect… • Don’t get to behind in studying for test 3 as well, it may come sooner than you realize

  3. simply answer the question. happy studying (if possible)!

  4. Lab 110 pts • What does PPE stand for?

  5. 10 pts • Personal protective equipment.

  6. Lab 120 pts • How long should the loop be leaved inside the incenerator?

  7. 20 pts • Until ¾ of the loop glows red hot

  8. Lab 130 pts • Which of the following is required PPE for MCB3020 lab? • Lab coat • Goggles • Gloves • Long sleeved shirts

  9. 30 pts • Long sleeved shirts

  10. Lab 140 pts • What BSL is the microbiology lab at UCF?

  11. 40 pts • BSL-2

  12. Lab 150 pts • First aid kits are located where?

  13. 50 pts • In the hallway between the labs.

  14. Lab 210 pts • True or False. When holding a microscope, you only need to hold it at the arm.

  15. 10 pts • False. You need to hold the arm and the base.

  16. Lab 220 pts • ____________ is a specialized staining method that uses an anionic dye to stain background rather than the cell.

  17. 20 pts • Negative staining

  18. Lab 230 pts • Nigrosin is an example of what sort of dye?

  19. 30 pts • Anionic dyes

  20. Lab 240 pts • Bacteria that exhibit a variety of cell shapes in a sample are known as ____________?

  21. 40 pts • Pleomorphic

  22. Lab 250 pts • Tetracocci are to tetrads as Sarcinae are to what?

  23. 50 pts • Cuboidal packets

  24. Lab 310 pts • The primary dye in the gram stain is?

  25. 10 pts • Crystal Violet

  26. Lab 320 points • What is the mordant used in the Gram stain?

  27. 20 points • Gram’s Iodine

  28. Lab 330 pts • Paulos is performing a Gram stain. He uses the primary dye but gets distracted and forgets to complete the rest of the steps. What color would the bacteria appear to be under his microscope?

  29. 30 pts • Purple

  30. Lab 340 pts • The Gram stain is a __________ stain.

  31. 40 pts • Differential

  32. Lab 350 pts • What is the decolorizer in the Gram stain? • dH2O • 70% Ethanol • H2O • 95% Ethanol

  33. 50 pts • 95% Ethanol

  34. Lab 410 PTS • Methylene blue will dye cells what color?

  35. 10 pts • Blue

  36. Lab 420 pts • True or False. Steaming is required after the secondary dye is used to stain the cell in Acid-Fast stain.

  37. 20 pts • False.

  38. Lab 430 pts • What is the decolorizer in the endospore stain?

  39. 30 pts • Water

  40. Lab 440 pts • Ziehl-Neelsen uses what dye as it’s primary dye?

  41. 40 pts • Carbolfuchsin.

  42. Lab 450 pts • Which of the following is matched correctly for the Schaeffer-Fulton Method? • Malachite green and Methylene blue • Carbolfuchsin and Safranin • Malachite green and Safranin • Carbolfuchsin and Methylene blue • Crystal violet and Safranin

  43. 50 pts • Malachite green and Safranin

  44. Lab 510 pts • The solid, gelatin-like substance used in petri dishes is known as what?

  45. 10 pts • Agar

  46. Lab 520 pts • True or False. You need to sterilize your loop when streaking from different zones on the same petri dish.

  47. 20 pts • True.

  48. Lab 530 pts • In which of the zones on a streak plate should you find isolation? • Zone I • Zone II • Zone III • Zone IIIB

  49. 30 pts • Zone IIIB.

  50. Lab 540 pts • What would the dilution factor be? 1 ml 7 ml 9 ml

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