slide1 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Avenanthramides in Oat Mitchell L. Wise USDA, ARS Cereal Crops Research Madison, WI PowerPoint Presentation
Download Presentation
Avenanthramides in Oat Mitchell L. Wise USDA, ARS Cereal Crops Research Madison, WI

Loading in 2 Seconds...

play fullscreen
1 / 19

Avenanthramides in Oat Mitchell L. Wise USDA, ARS Cereal Crops Research Madison, WI - PowerPoint PPT Presentation


  • 194 Views
  • Uploaded on

Avenanthramides in Oat Mitchell L. Wise USDA, ARS Cereal Crops Research Madison, WI. OUTLINE OF PRESENTATION. Phenylpropanoid/avenanthramide biosynthesis Health benefits of avenanthramides Application of suspension cultures to investigate avenanthramide biosynthesis.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Avenanthramides in Oat Mitchell L. Wise USDA, ARS Cereal Crops Research Madison, WI' - derora


Download Now An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1

Avenanthramides in Oat

Mitchell L. Wise

USDA, ARS

Cereal Crops Research

Madison, WI

outline of presentation
OUTLINE OF PRESENTATION
  • Phenylpropanoid/avenanthramide biosynthesis
  • Health benefits of avenanthramides
  • Application of suspension cultures to investigate avenanthramide biosynthesis
general phenylpropaniod pathway
General Phenylpropaniod Pathway

Glycolysis

pentose phosphate

+

phosphoenolpyruvate

erythrose-4-phosphate

Protein

Tryptophan/protein

Shikimate pathway

Tyr + Phe

Anthranilate

PAL

Phenylpropanoids

phytoalexins

phenolic

acids

lignins

flavonoids

reaction of phenylpropanoid and 5 hydroxyanthranilic acid catalyzed by hht
Reaction of Phenylpropanoid and 5-Hydroxyanthranilic Acid Catalyzed by HHT

Form R phenylpropanoid

A (Bp) H p-Coumaric

B (Bf) OH caffeic

C (Bc) OCH3 ferulate

grain avenanthramide content shows strong genotype x environment interaction
Grain Avenanthramide Content Shows Strong Genotype X Environment Interaction

Emmons & Peterson, Crop Sci 41:1676 (2001)

grain avenanthramide content shows strong genotype x environment interaction1
Grain Avenanthramide Content Shows Strong Genotype X Environment Interaction

Emmons & Peterson, Crop Sci 41:1676 (2001)

effect of avenanthramide c on vsmc proliferation
Effect of Avenanthramide C on VSMC Proliferation

[3H] thymidne incorporation (% of control)

Nie, Wise, Peterson, Meydani, Atherosclerosis, 186, 260-266 (2006).

avn c inhibition of restinoblastosis protein pp rb polyphosphorylation in vsmc
Avn C inhibition of Restinoblastosis protein (pp-Rb) polyphosphorylation in VSMC

Nie, Wise, Peterson, Meydani, Free Rad. Biol. Med, in press

effect of dietary avenanthramide c on exercise stressed rats
Effect of Dietary Avenanthramide C on Exercise Stressed Rats

Reduction of oxidized glutathione levels and higher ratio of reduced to oxidized form of glutathione.

O’Moore and Ji, (2006) unpublished results.

summary of health effects
Summary of Health Effects
  • Reduces vascular smooth muscle cell proliferation
  • Increased NO production
  • Inhibition of ppRb, arrest G1-S1 cell cycle progression
  • Elevation in anti-oxidant enzyme system in rats
northern analysis of suspension cells for barley pr 1 and hht subsequent to elicitation
Northern Analysis of Suspension Cells for Barley PR-1 and HHT Subsequent to Elicitation

18S RNA

bPR-1

HHT

conclusions future directions
Conclusions/Future Directions
  • Avenanthramides are a “value added” phytonutrient unique to oat
  • Avn content in the grain is highly variable
  • Basis for variability is unknown
  • Suspension cultures offer a model to study avenanthramide biosynthesis at the molecular level
  • SSH cDNA library prepared
  • What is the involvement of the various isozymes (HHT, 4-CL, PAL)?
acknowledgements
Jean Mayer Human Nutrition Research Center

MOHSEN MEYDANI

LIN NIE

Univ Wisconsin, Dept Kinesthesiology

LI LI JI

KATHLEEN O’MOORE

Cereal Crops Research

RACHEL ANGEL

LAURI HERRIN

NICKOLAS PARTRIDGE

RON SKADSEN

JOHN HERBST

ACKNOWLEDGEMENTS