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Carbon Monoxide Dehydrogenase Activity in Bradyrhizobim japonicum

Carbon Monoxide Dehydrogenase Activity in Bradyrhizobim japonicum. 이정은. Introduction. Carbon monoxide (CO) dehydrogenases (CODHs) - a key enzyme in the CO metabolism - carboxidotrophic bacteria - catalyzes the oxidation of CO to CO 2 CO + H 2 O -> CO 2 +2e - +2H +

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Carbon Monoxide Dehydrogenase Activity in Bradyrhizobim japonicum

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  1. Carbon Monoxide Dehydrogenase Activity in Bradyrhizobim japonicum 이정은

  2. Introduction • Carbon monoxide (CO) dehydrogenases (CODHs) - a key enzyme in the CO metabolism - carboxidotrophic bacteria - catalyzes the oxidation of CO to CO2 CO + H2O -> CO2 +2e- +2H+ - contains 1) molybdopterin 2) [2Fe – 2S] • Bradyrhizobium japonicum species - gram-negative soil bacteria - N2- fixing symbiosis - utilize H2 and CO2 as a sole sources of energy and carbon - use CO as a a sole sources of energy and carbon

  3. Results

  4. CO- dependent growth

  5. Purification and properties of CODH

  6. Metal contents and analysis of flavins and carboxamidomethylated proteins

  7. Discussion • B. japonicum 110spc4 - grew under CO-autotrophic condition. - hydrogen bacteria - utilize H2 and CO2 as a sole sources of energy and carbon - use CO as a a sole sources of energy and carbon - contain a CODH enzyme • CODH in 110 spc4 - NBT/ PMS - value of CO-oxidizing activity - nitrate reductase activity - heterohexameric subunit (LMS)2 - 75-kDa polypeptide /Cox L subunit of the O.carboxydovorans (immunological relation)

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