Evaluation of HLA-DR and Class I Antigen Expression in Ma-Mel-48 Cell Lines Treated with IFN-γ
This study investigates the expression of HLA-DR and HLA class I antigens in Ma-Mel-48 cell lines following treatment with IFN-γ (500 U/ml) for 48 hours. Control groups remained untreated. Cells were stained with monoclonal antibodies L243 and W6/32 to detect HLA-DR and HLA class I expressions, respectively. The findings, presented in Supplementary Figure S2, illustrate representative histograms from one of three independent experiments, demonstrating the modulation of antigen expression in response to IFN-γ treatment.
Evaluation of HLA-DR and Class I Antigen Expression in Ma-Mel-48 Cell Lines Treated with IFN-γ
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Presentation Transcript
Ma-Mel-48a Ma-Mel-48b Ma-Mel-48c + IFN-, sec mAb only w/o IFN-, sec mAb only + IFN- w/o IFN- HLA-DR HLA Class I HLA-DR (L243) HLA class I (W6/32) Supplementary Fig. S2:The different Ma-Mel-48 cell lines were treated with IFN- (500 U/ml) for 48 h, controls were left untreated. Cells were stained with mAb L243 and mAb W6/32 for detection of HLA-DR and HLA class I antigen expression, respectively. Representative histograms from one of three independent experiments are depicted.
