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Recrudescent Acanthamoeba Keratitis Related to Persistent Contact Lens Wear. Elmer Y Tu 1A , Charlotte E Joslin 1 , Megan E Shoff 2 , Janet A. Lee 1 , Ali Djalilian 1 1 Department of Ophthalmology and Visual Sciences University of Illinois Eye and Ear Infirmary Chicago, Illinois
Elmer Y Tu1A, Charlotte E Joslin1, Megan E Shoff2, Janet A. Lee1, Ali Djalilian1
1Department of Ophthalmology and Visual Sciences
University of Illinois Eye and Ear Infirmary
2Department of Molecular Genetics
The Ohio State University
1A Allergan – travel honoraria
No conflicts of interest pertaining to this presentation.
Off-label use will be discussed
Methods: Acanthamoeba keratitis patients diagnosed between 06/2003 – 09/2007
Partial immune ringCase 1
Bright-centered Acanthamoeba cysts
Diff-Quik Stain (Original magnifiction 100x oil)
Sequence comparison of the two isolates obtained from Case 2 for a 135 nucleotide base region within one of the highly informative diagnostic fragments of the Acanthamoeba 18S rRNA gene. The two isolates, 06-005 and 07-072, clearly differ from each other, with 13/135 base differences. However, as shown, each isolate separately closely resembles different previously reported Acanthamoeba sequences found in the DNA database GenBank.
Joel Sugar, MD
Leslie T. Stayner, PhD
Jamie Brahmbhatt, COMT
The Ohio State University, Dept. Molecular Genetics
Gregory C. Booton, PhD
Paul A. Fuerst, PhDAcknowledgments
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