TSLC(TUMOR SUPPRESSOR IN LUNG CANCER)1 SUPPRESSES EPITHELIAL CELL SCATTERING AND TUBULOGENESIS Mari Masuda, et. al. (2005) J. Biol. Chem. 280, 42164-42171. 銘傳大學生物科技學系 姓名 : 尹馭群 學號 :91390576. Introduction. TSLC1 EMT Cyst Cell adhesion molecules Epithelial tissue and connective tissue
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TSLC(TUMOR SUPPRESSOR IN LUNG CANCER)1 SUPPRESSES EPITHELIAL CELL SCATTERING AND TUBULOGENESISMari Masuda, et. al. (2005)J. Biol. Chem. 280, 42164-42171
Epithelial cells are the cells that line virtually every organ in your body and 85% of cancers derive from epithelial cells. During embryonic development epithelial cells sometimes dissolve their junctions with their neighbors and become mesenchymal. Mesenchymal cells have a less rigid shape and are more likely to be motile. Epithelial to mesenchymal transitions, as well as the reverse process, are extremely important for normal development. In addition, these transitions are important in wound healing, and tumor cells that develop from epithelial cells must transform into motile cells in order to metastasize.
(Rho, Rac )
1.The obtained clones express the transgenes at a nearly equivalent level
2. MDCK Tet-off cells express a low level of endogenous TSLC detected as faint bands
Overexpression of TSLC or any of the truncation mutants does not alter cell growth in MDCK cell
Quantitativeanalysis of 30 cysts for each cell clone demonstrated that the differencein tubulogenesis between the Dox-treated (TSLC1 off) and untreated(TSLC1 on) MDCK/TSLC1 cells was statistically significant (p < 0.0001), whereas other cell clones showed no statistically significant difference between the Dox-treated and untreated cysts (p>0.05)
A quantitative analysis of cell scattering confirmed that the difference between the Dox-treated and untreated MDCK/TSLC1 cells was statistically significant with p <0.0001. In contrast, other cell clones showed no significant difference between the Dox-treated and untreated cells (p>0.05)
In response to HGF, the parental cells showed immediate early increase in Rac activity,which returned to basal levels within 15 min as well, as sustained Rho activation