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Study on apoptotic nuclei increase post 5 Gy X-ray irradiation. Time-dependent changes in cell number analyzed. Results compared between control and irradiated cells. Statistical significance shown (p < 0.01).
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A B 100 20 ** ** ** ** ** ** Relative cell No. ** 50 ** % Apoptotic nuclei 10 ** ** ** 0 0 5 7 2 3 4 0 1 5 5 7 2 3 4 0 1 5 Days after X-rays Days after X-rays Apoptotic cell death caused by 5 Gy of X-rays. A, Time-dependent increase in apoptotic nuclei, which were detected by homogeneous nuclear staining by anti-phosphorylated histone H2AX antibody. B, Time-dependent change in the cell number, which was determined as described in Materials and Methods. The data represent the mean ± SEM. Significance was evaluated between the control and irradiated cells. **p < 0.01. Figure S2