Binary Ionization Technology Systems

1. Binary Ionization Technology Systems H2O2 SolutionsLLC This technical data is controlled under the U.S. International Traffic in Arms Regulations (ITAR) and may not be exported to a Foreign Person, either in the U.S. or abroad, without the proper authorization by the U.S. Department of State.

2. Binary Ionization Technology Systems BIT Plasma Head • Energized Chemistry - H2O2 Ionization: Cold Plasma (BIT Plasma) UV Light, heat, ozone Chemical Activation (BIT Chemical) • Basic Chemical Reaction: H2O2 + activator peroxy acids + hydroxyls, reactive oxygen species (ROS) Redox potential greater than chlorine or peroxide alone • What it Does: Destroys proteins on contact (via oxidation) killing biological agents including viruses, molds, bacteria and bacterial spores Reacts with chemical bonds in chemical agents destroying their activity BIT Mobile Control Units

3. What is the BIT Plasma System? • Aerosolized solution containing hydrogen peroxide • Electric arc produced by 17K volt potential • Hydrogen peroxide, nitrogen and oxygen are energized (ionized) • Creates a variety of “free radicals” including: Hydroxyl radical Hydroxyl ion Superoxide anion Singlet oxygen Other reactive oxygen species (ROS)

4. Reactive Oxygen Species (ROS) Destroy Chemical Bonds, Disrupting Proteins, Lipids and Chemicals VX GA DPCP

5. BIT Plasma System Commercial Product • SteraMist Fogging Unit Primary use is to decontaminate surfaces in tissue banks, hospitals and pharmaceutical manufacturing facilities Extensive in-house and 3rd party testing against a wide range of pathogens Production of commercial units and sales began in September 2008 Use of extendable ‘pods’ allow for flexibility of mist application

6. Extensive Testing Against Biologicals • In-house and multiple 3rd party tests Exposure times 15-90 seconds Killing up to 9 logs Effective against mold, spores, viruses and bacteria

7. BIT PlasmaAdvantages • Broad spectrum including bacterial and fungal endospores • ROS kill by lysis of cell walls • Rapid cycle time • Environmentally friendly-residue H2O+O2 • H2O2 concentration of 7.5% is safe for personnel • No damage to interior materials • Electrostatic spray extensive distribution • Log kill organisms >6 for all organisms tested

8. How Common Is MRSA? • Jarvis, et al,”National Prevalence of MRSA in Inpatients in Health Care Facilities,2006,” AJIS, vol35#10, 12-07 • P > 46.3/1000 inpatients ( 34 infect and 12 colonization /1000 inpatients )

9. The Value of a Clean Environment • Huang et al, “The Risk of Aquiring Antibiotic Resistant Bacteria From Prior Room Occupants,” Arch of Int Med, vol 166#18, 10-06 • Drees et al,”Prior Environmental ContaminationIncreases the Risk of Acquisition of Vancomycin-Resistant Enterococci,” Clin Infect Dis, vol 46 #2, 3-08 • Otter et al,”Survival of Nosocomial Bacteria and Spores on Surfaces and Inactivation by H2O2 Vapour,”J Clin Microbiol, vol47#2, 2-09

10. How Important is the Environment • Harris, Anthony, “Editorial: How important is the environment inj the Emergence of Nosocomial Antibiotic Resistant Bacteria?,” Clin Infect Dis, vol 46#2 3-08 • While patient to patient transmission via health care workers is an important mechanism, the role of environmental contamination appears significant

11. Does Disinfection Reduce Risk? • Deterkofer et al,” Does Disinfection of Environmental Surfaces Influence Nosocomial Infection Rates? AIIC vol 32 #2, 4-04

12. Are Conventional Techniques Adequate? • Carling et al,”Intensive Care Unit Environmental Cleaning: An Evaluation of Sixteen Hospitals Using a Novel Assessment tool,” J of Hosp Infect, vol 68, 1-08 • Goodman et al,”Impact of Environmental Cleaning Intervention on the Presence of MRSA and VRE on Surfaces in Intensive Care Unit Rooms,” Infec Control and Hosp Epi, vol29#7, 7-08

13. Is Infection Control Cost Effective? • Perencevich et al, “Raising Standards While Watching the Bottom Line: Making a Business Case for Infection Control,” Infect Control and Hosp Epi, vol 28#10, 10-07

14. Is H2O2 Decontamination Effective, Safe, and Practical? (Steris ) • Boyce,John M.,”New Approaches to Decontamination of Rooms,” Infec Control and After Patients Are Discharged Hosp Epi, vol30#5 6-09

15. Charged Aerosol In or outside No special Tx Surface activity No prep Active immediately OK with HVAC Redox =2.8 (fl=3.0) Need optimal -dry-conditions Inside No surface activity Hours to prep Needs 30’ exposure Corrodes HVAC duct Redox H2O2 =1.8 BIT Plasma H2O2 vs Steris-VHP

16. Spread enhanced by charged particles Std bio indicator Rapidly H2O +O2 Safe with metals H2O2 conc 7% is non toxic ( except eye) Fabric/cellulose OK Limited penetration b/o moisture effect Special SS indicator Slow decomp ,Hard to monitor Oxidizes metals H2O2 conc 50%-toxic to lungs skin eye Fabric-cellulose separate decontam BIT Plasma vs Steris VHP

17. Verification • 3M Clean Trace ATP System • Biological Indicators: Geobacillus stearothemophilus (incubated at 55-60 degrees C, in Trypticase Soy Broth)

18. H2O2 Solutions • Sterilization Services • BIT SteraMist Sales

19. Objective - Services • To drastically reduce the index and related costs of hospital-acquired infections by efficiently sterilizing the areas where dangerous pathogens are normally found.

20. Advantages • Reduction of operational costs (room turn-around times). • Reduction of functional costs (changes in Medicare). • Reduction in exposure to litigious events. • More active protection of patients, staff, and visitors.

21. H2O2 Services • Proven technology - highly effective, quick to deploy and utilize, no toxic or harmful by-products. • Inclusive of all equipment and materials. • Knowledgeable, flexible staff. • Committed, long term relationship. • Turn-key operation. • 24/7 “on-call” availability.

22. Turn-Key Operation • Site survey. • Functional qualification of each area. • Tailored to customer’s schedule. • Process repeatability and quality control. • Documented process and results. • Record keeping. • 24/7 “on-call” availability