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C. fern. AP Biology. Ferns. Alternation of Generations. C. Fern sporophyte. Aseptic Technique. ASEPTIC TECHNIQUE Wipe the laboratory desk counter with Clorox solution( 10% bleach) using paper towels.

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c fern

C. fern

AP Biology

aseptic technique
Aseptic Technique
  • ASEPTIC TECHNIQUE
  • Wipe the laboratory desk counter with Clorox solution( 10% bleach) using paper towels.
  • Use rubbing alcohol full strength from the supermarket (70%) in a clearly marked plant misting bottle to spray the inside surfaces of your hood. Allow to dry for five – ten minutes. The surface will be disinfected
  • Wash your hands thoroughly. Do not touch anything before working in your hood.
  • Place a beaker containing alcohol in your hood. Soak your spreader in the beaker. Disinfect any tools you are going to use before preparing your culture. ( scalpels, forceps, probes)
  • Use sterile pipets or pipet tips.
  • Keep the front of your hood partially closed.
  • Do not talk or chat while you work. Do not cough or sneeze into the hood.
  • Work slowly to keep the air still.
  • Only open the top of your culture dish when you are ready to spread your spores on the culture or prepare your plant specimens for culture.
  • P. Sidelsky. Revised from the Fralin Biotechnology Center Protocol, Virginia Tech