Anaerobic Culture Techniques. 3rd Science Practical Seminar 1 Vincent O’Flaherty. Anaerobic microorganisms are widespread and very important Do not require oxygen for growth - often extremely toxic. Facultative anaerobes - can grow in the presence or absence of oxygen
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3rd Science Practical Seminar 1
Oxygen tolerance of bacteria is dependent on which of these enzymes they possess.
- 300 mV
Grows aerobic or anaerobic.
2. Place 4.5ml of pre-reduced anaerobic agar medium into tube
3. Seal the tube with the butyl rubber stopper and screw cap
4.Autoclave the tube
5.Inoculate with a syringe
6.Prepare on roll tube spinner
7.Incubate in water bath