Python Programming on PCR Primers Design. Ronny Chan SoCalBSI July 28, 2004. Polymerase Chain Reaction. PCR is a technique that is used to amplify a sample of DNA from miniscule amount of DNA (ex., DNA from a crime scene, archaeological samples, organisms that can’t be cultured).
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July 28, 2004
Input the length of primers
Input DNA sequence
Input the start and end of central region
GC content 45-55%
Hairpin and self-dimerization
List of acceptable primers