PowerPoint Slideshow about '嚼食檳榔的口腔癌患者中第一型基質金屬蛋白酶 ; 的表現' - cutter
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The squamous cell carcinoma (SCC) rate is the highest (95%) among oral cancers. About 80-90% of the patients of oral cancer chew betel nuts for a long time. Most ingredients of the nuts may hurt the oral mucosa mechanically and chemically. Betel nut chewing has been proved to cause epithelial atrophy, hyperkeratosis, even oral cancer. Matrix metallo- proteinases (MMPs), a group of zinc-dependent endopeptidases, involve extracellular matrix (ECM) physiological degradation process. Moreover, MMPs influence other pathological processes of diseases. During the tumor invasion, cancer cells break through the basement membrane and enter connective tissue, which is a crucial basic characteristic, and varies with the changes in the interaction of cells, matrix and matrix degrading enzymes. Among the enzymes, MMPs is one of the most important. During tumor cell proliferation, the adjustment between extracelluar matrix and MMPs is important. MMPs can remodel tumor cells of peripheral tissue and be induced in the expression during the interaction among tumor cell, ECM, and inflammatory cell. Thus MMPs are considerably important in tumor tissue remodeling, repairing and destroying. Thus the up- and down- expression of MMPs is significantly different when applied to predict tumor cell invasion and transfer. The overexpression of some MMPs were associated with oral cancer occurrence, proliferation, lymph node metastasis and prognosis in recent studies. Matrix metalloprotinase-1 (MMP-1) cleave type I, II and III collagen in connective tissues. The overexpression of MMP-1 is associated with oral cancer occurrence, proliferation, lymph node metastasis and prognosis in recent studies. MMP-1 is considerably important in degrading of ECM. Thus the up- and down- expression of MMP-1 is significantly different when applied to predict tumor invasion and prognosis. But the overseas MMP-1-associated cases of oral cancer lacked those of Taiwan, where there are many betel nut chewers. Therefore, this research aimed at Taiwan, to analyze the MMP-1 expression of oral cancer in the just- mentioned patients. The Department of Oral and Maxillofacial Surgery of Chi-Mei Medical Center especially studies 30 oral cancer patients of betel nut chewing and about the associated MMP-1 expression and relation. In RT-PCR analysis, we found that MMP-1mRNA was overexpressed in all the tested 30 OSCC. In Western blotting analysis, levels of MMP-1 protein was significantly elevated in the tested OSCC specimens than in their adjacent tissues. In IHC analysis, MMP-1 expression is also higher in the epithelium of tumor tissues. Esophageal carcinoma (CE81T/VGH) and OSCC (OECM-1) cell lines survived from the cytotoxic BQ extract (BQE) and arecoline selection process were found to express higher MMP-1 mRNA. These reports proved the significant effect of MMP-1 in betel nut chewing associated oral cancer, tumor growth, tumor differentiation and lymph node metastasis.