1 / 4

TRPV5 Gene Organization and Constructs: Promoter Study in Mouse Kidney Cells

This study delves into the genomic organization of the TRPV5 gene with emphasis on the TRPV5 promoter region, including constructs like pAcGFP1-N1 plasmid, elucidated through detailed flow charts and immunolabeling in various kidney segments such as DCT, CNT, and CCD. The examination extends to the development of DCT2/CNT-specific AQP2 knockout mice. This comprehensive analysis sheds light on the intricate regulation and expression of relevant genes in renal tubules.

curt
Download Presentation

TRPV5 Gene Organization and Constructs: Promoter Study in Mouse Kidney Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fig. 15.1 Genomic organization of the TRPV5 gene and constructs. (a) The TRPV5 promoter (orange box) consists of a 3.6 kb region between the TRPV5 and TRPV6 genes on mouse chromosome 6. Peaks represent calculated Gerp intraspecies conservation scores. (b) Simplified map of the final pAcGFP1-N1 plasmid Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer

  2. Fig. 15.2 Flow chart of the validation of the TRPV5 promoter driven EGFP expressing transgenic mouse line Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer

  3. Fig. 15.3 TRPV5 promoter-driven EGFP expression in DCT2, CNT, and CCD. (a) Tripleimmunolabeling confocal microscopical analysis of fixed kidney sections for calbindin-D28 K, Na+-Cl– cotransporter (NCC), and EGFP. (b) Similar immuno-fluorescence labeling for aquaporin-2 (AQP2), NCC, and EGFP. (c) Immuno-fluorescence labeling for vacuolar type proton pump (H+-ATPase), AQP2, and EGFP. (d) Schematic representation of the renal tubular system with indication of the tubule segments and expression sites for EGFP, NCC, calbindin-D28 K, AQP2, and H-ATPase Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer

  4. Fig. 15.4 (a) Map of the pBS185 plasmid containing the TRPV5 promoter fragments, including restriction sites. (b) Flow chart of the development of DCT2/CNT-specific AQP2 knockout mice Transient Receptor Potential Channels, Md. Shahidul Islam (Ed.) ISBN: 978-94-007-0264-6, Springer

More Related