Measurement Of Haematocrit (PCV). Dr Maliha Sumbul. Haematocrit:. Relative volume of blood occupied by erythrocytes. An average figure for humans is 45ml per cent, i.e. A packed red cell volume of 45ml in 100ml of blood. PCV / Packed cell Volume / Haematocrit.
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Dr Maliha Sumbul
High Speed Haematocrit centrifuge
Groups of individuals who are at risk for developing anemia include:
40 – 55% for men
REAGENTS AND EQUIPMENT:
2 types – heparinized tube (red band)
-plain tube with anticoag. WB
( blue band )
The microhaematocrit tube holds approx.
0.05 ml of whole blood
Reagents and Equipment - continued inadvertently drawn
( The liquid tripot. salts of EDTA can cause 2-3% decrease in haematocrit due to shrinkage of rbcs )
(air bubbles denote poor technique but do not affect the results)
( make certain that blood is not forced out during the process)
( PARALLAX: defined as an object being seen in a different position by changing the position of the head, or as seen by one eye versus the other eye)
apply white tape to the inner side
- usually expressed as a % of the red blood cell
- 1-3% higher b/c of trapped plasma when compared with automated cell counter
- Increased amount of trapped plasma is found in: macrocytic anemias, spherocytosis, thalassemia, hypochromic anemias and sickle cell anemia
QUESTIONS ? centrifuged within 6 hrs of collection when blood is stored at room temperature