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Normal Flora

Normal Flora. Microbiotica. Microbiotica. Synder Deep. CH2O fermentation Glucose Differential Rate of change susceptibility Selective for lactobacillus pH = 4.8 Indicator = bromcresol green Dental caries. BAP. Divide Plate Swab throat Differential Sheep Blood Hemolysis

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Normal Flora

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  1. Normal Flora Microbiotica

  2. Microbiotica

  3. Synder Deep • CH2O fermentation • Glucose • Differential • Rate of change • susceptibility • Selective for lactobacillus • pH = 4.8 • Indicator = bromcresol green • Dental caries

  4. BAP • Divide Plate • Swab throat • Differential • Sheep Blood • Hemolysis • Hemolysin exotoxins • Types • Gamma • Alpha • Beta

  5. Chocolate Agar • Divide Plate • Swab throat • Differential • Growth • Oxidase test • Neisseria • Lysed RBCs

  6. MSA • Selective • Differential • Divide plate • Swab Skin • Nose • Face • Results • Fermentation • acid end products • yellow • Components • 7.5% NaCl • mannitol • pH indicator = phenol red

  7. MH-T • Divide plate • Swab skin • Ears • Palms • Differential • Diptheroids • Tellurite • Reduced to tellurium • Black (grey) colonies

  8. Nutrient Agar • TSA • Not selective • Not differential • Divide plate • Swab environment • Moisten swab • Label source

  9. Sabourauds • Selective • Yeast • Fungus • +/- Differential • Acidic pH • Divide plate • Swab environment • Moisten swab • Label source

  10. Urine Culture • TSA plate • Calibrated loop (0.01ml) • Clean catch sample • Swab plate • Confluent • Isolation • Calculate CFU/ml

  11. Normal Flora Results

  12. Snyder Deep Results • CH2O fermentation • Glucose • Differential • Rate of change • Susceptibility for caries • Selective for lactobacillus • pH = 4.8 • Indicator = bromcresol green • Green  Yellow • Dental caries • Slight • Mild • Moderate • Severe

  13. BAP results • Not selective • Differential • Hemolysis • Hemolysin exotoxins • Types • Gamma • Alpha • Beta

  14. Chocolate Agar Results • Differential • Growth • Lysed RBCs • CO2 = capnophiles • Oxidase Test • Neisseria

  15. Oxidase Test • Oxidase Reagent • Cytochrome C in ETC • Dark Purple = positive • Neisseria

  16. Oxidase Reaction

  17. MSA Results • Selective • Differential • Results • Fermentation • acid end products • yellow • Components • 7.5% NaCl • Mannitol • pH indicator = phenol red

  18. MH-T Results • Differential • Diptheroids • Mueller Hinton • Nutrient agar • Controlled proteins/ions • Diffusion • Tellurite • Reduced to tellurium • Black (grey) colonies

  19. Sabarouds Results • Selective • Yeast • Fungus • +/- Differential • Acidic pH • 5.5 • acidophiles

  20. TSA Environmental Culture

  21. UTI • CFU/ml • # colonies/0.01ml • UTI • = > 1x10(5)/ml • Clinical signs • Other urine tests

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