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CULTIVAREA SI IDENTIFICAREA VIRUSURILOR. Structura foarte simpla agenti infectiosi acelulari contin acizi nucleici si invelis proteic protector sunt adaptati la un parazitism obligator intracellular se pot cultiva doar in celula vie. VIRUSURILE. PARAZITISM ABSOLUT AL CELULELOR VII

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Presentation Transcript
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Structura foarte simpla
    • agenti infectiosi acelulari
  • contin acizi nucleici si invelis proteic protector
  • sunt adaptati la un parazitism obligator intracellular
  • se pot cultiva doar in celula vie
virusurile
VIRUSURILE
  • PARAZITISM ABSOLUT AL CELULELOR VII
  • SISTEME CELULARE

- ANIMALE DE EXPERIENTA

- OUA EMBRIONATE

- CULTURI DE CELULE

virusurile1
VIRUSURILE
  • EVIDENTIEREA VIRUSURILOR

- EXAMINAREA MICROSCOPICA A

PRODUSELOR PATOLOGICE SI A

TESUTURILOR INFECTATE

(MODIFICARI SPECIFICE VIRUSURILOR)

- CULTIVAREA SI IDENTIFICAREA PRIN METODE SPECIFICE

  • DEMONSTRAREA UNEI CRESTERI A TITRULUI ANTICORPILOR SPECIFICI (FAZA ACUTA/ CONVALESCENTA)
cultivarea virusurilor inocularea la animalele de experienta
CULTIVAREA VIRUSURILORINOCULAREA LA ANIMALELE DE EXPERIENTA
  • SUNT UTILIZATE ANIMALE MICI
  • EXISTA DIFERENTE DE SENSIBILITATE

- SPECIE

- RASA

- VARSTA

cultivarea virusurilor caile de inoculare
CULTIVAREA VIRUSURILORCAILE DE INOCULARE
  • SUBCUTANATA
  • INTRAVENOASA
  • INTRANAZALA
  • INTRAPERITONEALA
  • SCARIFICARE CUTANATA SAU CORNEEANA
  • INOCULARE INTRACEREBRALA
cultivarea virusurilor caile de inoculare1
CULTIVAREA VIRUSURILORCAILE DE INOCULARE
  • REPRODUC MODALITATEA DE PATRUNDERE A VIRUSULUI IN ORGANISMUL UMAN
  • ARTIFICIALE
cultivarea virusurilor demonstrarea replicarii virale
CULTIVAREA VIRUSURILORDEMONSTRAREA REPLICARII VIRALE
  • LEZIUNI

MACROSCOPICE/ MICROSCOPICE

  • REPRODUCTIBILITATEA LEZIUNILOR
virusurile coxsackie
VIRUSURILE COXSACKIE
  • INOCULARE SUBCUTANATA LA SOARECELE SUGAR

- COXSACKIE A - PARALIZIE FLASCA

- MIOZITA GENERALIZATA

- COXSACKIE B – PARALIZIE SPASTICA

- MIOZITA LOCALIZATA

- LEZIUNI SNC SI VISCERE

virusurile coxsakie
VIRUSURILE COXSAKIE

VEZICULE

BOALA

MANA-PICIOR-GURA

virusurile gripale
VIRUSURILE GRIPALE
  • INSTILARE INTRANAZALA LA SOARECELE ADULT
  • PNEUMONIE INTERSTITIALA CARACTERISTICA
gripa aviara
GRIPA…AVIARA

ARCA LUI NOE SI GRIPA AVIARA

virusurile herpetice
VIRUSURILE HERPETICE
  • SCARIFICARE CORNEANA LA IEPURE
  • APARITIA KERATOCONJUNCTIVITEI

KERATOCONJUNCTIVITA LA IEPURE DUPA SCARIFICARE CORNEEANA

virusurile herpetice1
VIRUSURILE HERPETICE

HERPES LABIAL

HERPES GENITAL

virusurile herpetice2
VIRUSURILE HERPETICE

NERV MAXILAR

NERV OFTALMIC

virusurile herpetice3
VIRUSURILE HERPETICE

NERVI INTERCOSTALI

virusurile herpetice herpes zoster
VIRUSURILE HERPETICEHERPES ZOSTER

VEZICULE LA NIVEL OROFARINGIAN

VEZICULE SI CRUSTE PE TRAIECTUL

N. OFTALMIC / MAXILAR

virusurile herpetice herpes zoster1
VIRUSURILE HERPETICEHERPES ZOSTER

HERPES ZOSTER INTERCOSTAL

virusul rabic
VIRUSUL RABIC
  • INOCULARE INTRACEREBRALA LA SOARECE
  • ENCEFALITA RABICA
  • HEMATOXILINA-EOSINA

CORPUSCULI BABES-NEGRI

virusul rabic1
VIRUSUL RABIC

CORPUSCULI INTRACITOPLASMATICI- BABES ERNST

cultivarea virusurilor inocularea in culturi de celule
CULTIVAREA VIRUSURILORINOCULAREA IN CULTURI DE CELULE

RECIPIENTE PENTRU CULTURI DE CELULE

VERIFICAREA PH-ULUI MEDIULUI DE CULTURA

cultivarea virusurilor inocularea in culturi de celule2
CULTIVAREA VIRUSURILORINOCULAREA IN CULTURI DE CELULE

INCUBATOR PENTRU CULTURILE DE CELULE

exemple de linii celulare stabilizate
MRC-5 Human lung Fibroblast

HELA  Human cervix Epithelial

VERO  African Green Monkey Kidney Epithelial

NIH 3T3Mouse embryo Fibroblast

L929Mouse connective tissue Fibroblast

CHO  Chinese Hamster Ovary Fibroblast

BHK-21Syrian Hamster Kidney Fibroblast

HEK 293Human Kidney Epithelial

HEPG2Human Liver Epithelial

EXEMPLE DE LINII CELULARE STABILIZATE
exemple de linii celulare stabilizate1
EXEMPLE DE LINII CELULARE STABILIZATE

BAE-1  Bovine aorta Endothelial

NSO  Mouse myeloma Lymphoblastoid-like

U937Human Hystiocytic LymphomaLymphoblastoid

Namalwa Human Lymphoma Lymphoblastoid

HL60  Human Leukaemia Lymphoblastoid-like

WEHI Mouse B-cell Lymphoma Lymphoblastoid

YAC 1 Mouse Lymphoma Lymphoblastoid

U 266B1 Human Myeloma Lymphoblastoid

SH-SY5Y  Human neuroblastoma Neuroblast

culturi de celule
CULTURI DE CELULE

A. CULTURA PRIMARA

B. CULTURA 16 ZILE

C+D

EVIDENTIERE PRIN IMUNOHISTOCHIMIE

A+B PRIMA GENERATIE

C+D A 2-A GENERATIE

A TESUT OSOS

SANATOS

B NODUL

CALCIFICAT

C PASAJUL 2

incluzii celulare intracitoplasmatice
INCLUZII CELULARE INTRACITOPLASMATICE

V. RABIC

INCLUZII INTRACITOPLASMATICE

incluzii intracitoplasmatice
INCLUZII INTRACITOPLASMATICE

V.EBOLA

INCLUZII INTRACITOPLASMATICE

HEPATOCITE

reactii antigen anticorp utilizate in virusologie
REACTII ANTIGEN-ANTICORP UTILIZATE IN VIRUSOLOGIE
  • EVIDENTIEREA ANTIGENELOR (VIRUSURILE)
  • EVIDENTIEREA ANTICORPILOR (CRESTEREA DE CEL PUTIN 4 X A TITRULUI)