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Cyclin B-GFP + AS

A. Cyclin B-GFP mRNA ± Mad2MO. GFP-targeting antisense (AS). GVBD. Metaphase II. PB. ? duration. Wash out of dbcAMP. Timelapse Imaging. Translation x 2-3 h in dbcAMP. GV. B. Cyclin B-GFP + AS. Cyclin B-GFP + Mad2MO + AS. Total oocyte fluorescence. Time (h post-GVBD). C.

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Cyclin B-GFP + AS

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  1. A Cyclin B-GFP mRNA ± Mad2MO GFP-targeting antisense (AS) GVBD Metaphase II PB ? duration Wash out of dbcAMP Timelapse Imaging Translation x 2-3 h in dbcAMP GV B Cyclin B-GFP + AS Cyclin B-GFP + Mad2MO + AS Total oocyte fluorescence Time (h post-GVBD) C Cyclin B-GFP + AS D GV 02:00 04:00 06:00 08:00 10:00 12:00 GV E Cyclin B-GFP + Mad2MO + AS F GV 02:00 04:00 06:00 08:00 10:00 12:00 GV Supplementary Fig 5_Homer et al.

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