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Broad-band Decoupling. Decoupled 1-D NMR Pulse Sequence. Basic 1-D NMR Pulse Sequence. 1-D NMR of a protein. 2-D NOESY of a protein. 1H/1H-COSY NMR. Mass Spectrometry. Chapters 11 and 20. Example Mass Spectrum. Dalton – amu Molecular ion peak

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Broad band decoupling
Broad-band Decoupling

Decoupled 1-D NMR Pulse Sequence

Basic 1-D NMR Pulse Sequence


Broad band decoupling

1-D NMR of a protein

2-D NOESY of a protein



Mass spectrometry

Mass Spectrometry

Chapters 11 and 20



Broad band decoupling

Dalton – amu

Molecular ion peak

– m/z corresponding to MW of singly charged molecule

Fragment peak

– m/z less than MW of singly charged molecule

Base peak

– m/z peak that is most intense


Broad band decoupling

Hard ion source

– leaves excess energy in ion

– extensive fragmentation

Soft ion source

– leaves less energy in ion

– reduced fragmentation