The Keys to Laboratory Safety: Semester Review

1. The Keys to Laboratory Safety: Semester Review Bill Diesslin March 3, 2009

2. U.S. Department of Labor Occupational Safety and Health Administration OSHA Chemical Safety in the LabRisk Management Name two federal agencies that have regulations that deal with chemical use in the laboratory.

3. Chemical Risk Management What is the name of the document that is the primary resource for information on how to use chemicals in laboratories at ISU?

4. ISU Laboratory Safety Manual • Introduction • Process Planning • Emergency Planning • Equipment • Training • General Lab Safety Practices • Safety Practices for Specific Hazards • Management of Unwanted Lab Materials (Waste) • Exposure Assessment and Medical Care • Appendix I: Forms • Appendix II: Lab Related Manuals

5. ISU Laboratory Safety Manual • YOUR RESPONSIBILITIES: • Familiarize yourself with the document • Be aware of lab hazards • Follow Standard Operating Procedures • Review processes with major professor • Use equipment properly (i.e., PPE, fume hoods, etc.) • Label, store & dispose of chemicals properly • Manage chemical inventories • Be a good example to others

6. Personal Protective Equipment Chemical-resistant gloves are commonly used in the laboratory. Name 3 things to be aware of when selecting and using chemical-resistant gloves.

7. Personal Protective Equipment • PPE is last line of defense • Identify the hazards • Selecting the right PPE • Types available • Minimum required in a lab • Special cases • Your responsibilities when wearing PPE • Regulated by OSHA • Part of SOPs

8. Chemical Hazard Information Name two sources of chemical safety information.

9. Chemical Hazard Information • Constituents of MSDS • Health & physical hazards • Controls • Label requirements – secondary containers • Relative to use • Allows hazard assessment

10. Chemical Hood Usage Name three things to keep in mind when using a chemical hood.

11. Chemical Hood Usage • Purpose • Operational Characteristics • Proper Hood Usage • Common Usage Problems • Servicing

12. Laboratory Inspections Names three things that would be deficiencies listed on a laboratory inspection form.

13. Laboratory Inspections POSITIVE IMPRESSIONS • Every occupant wearing PPE • Door has current emergency contact information and NFPA sign • No food or drink containers in laboratory • Room is clean and uncluttered • Eyewash station is clean and labeled as being tested • Chemical containers are from the 21st century • All glassware is clean and put away when not in use • All chemical containers has labels with names spelled out • Centralized location for all documentation (Laboratory Safety Manual, MSDSs, training records, emergency contact info)

14. Hazardous Waste Disposal The new term used to refer to waste is _________ ________.

15. Hazardous Waste Handling at ISU

16. Chemical Storage Peroxide-forming chemicals must be tested how many months after opening of the container?

17. Chemical Storage • Why is proper storage critical? • Labeling • General Storage Considerations • Storage in Refrigerators • Storage in Fume hoods • Flammable Storage

18. Hazardous Materials Shipping Name the U.S. authority for all shipments by land, sea and air.

19. Hazardous Materials Shipping • What materials are regulated for shipping? • What can happen if you don’t comply with the regulations? • What are the steps involved in shipping?

20. Biosafety What is the name for an organism that causes diseases in humans, plants or animals?

21. Biosafety • Recombinant DNA • Transgenic Animals or Plants • Bacteria, Viruses, Fungi, Prions • Biological Toxins (e.g.Tetanus Toxin) • Human Blood and Certain Body Fluids • Institutional Biosafety Committee approval

22. Emergency ActionsChemical Spill Procedures Name two things you would find in an emergency action plan.

23. Emergency ActionsChemical Spill Procedures • Types of emergencies • Procedures • Alarm systems • Chemical spill issues • Mercury • Small versus large? • Part of employee training

24. Electrical Safety Name two types of electrical shock injuries and two factors impacting electrical shock.

25. Electrical Safety • Improper use of electrical equipment. • Damaged equipment: cords, plugs. • UL-approved • GFCI • Vapors

26. Fire Safety in the Lab Name the three components of the fire triangle.

27. Fire Safety in the Lab • Fire Chemistry and Principles • Controlling Lab Fire Hazards • Fire Evacuation Procedures • Fire Extinguishers • PASS

28. Common State Fire Marshal Fire Safety Citations - ISU • Hallway doors opened • Storage < 18” below sprinkler heads • < 3’ clearance by electric panels • Improper use of extension cords & door wedges • Lack of orderly and clean laboratories

29. Pressure Safety As volume increases, pressure __________; as temperature increases, pressure __________.

30. Pressure Safety • MAWP • Safety Factor • Hazards of cryogens? • Cylinders: primary/secondary hazards

31. Radiation Safety Radiation that has enough energy to cause the release of an orbital electron is called __________.

32. Radiation Safety • Radiation Types • Ionizing • Non-Ionizing • Controls • ALARA

33. Laser Safety Name 3 ways that you can prevent exposure to a laser beam.

34. Laser Safety • “LA-SER” • Which classes of lasers are hazardous? • Which non-beam hazard is the most significant? • How do you protect yourself when using a laser?

35. That’s It!