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  1. Lab Meeting July 12, 2011

  2. FFPE sample Protein Extraction 6 5 7 Lanes 4 1 3 2 1 2 3 4 5 6 7 Mrker Lane 1: Shed Cells (FOP sample) ~25µg Lane 2: X Lane 3: HB09-1 Lane 4: HB09-8 Lane 5: HB09-9 Lane 6: HB09-11 Lane 7: HB08-20 Mrker Lane 1: Shed Cells (FOP sample) ~13µg Lane 2: X Lane 3: HB09-6 Lane 4: HB09-7 Lane 5: HB09-9 Lane 6: HB08-19 Lane 7: HB08-20

  3. Comassie Stained Gel 6 8 5 7 Lanes 4 1 2 3 Mrker Lane 1: Shed Cells (Norm sample) ~20µg Lane 2: HB09-10 Norm Lane 3: HB09-1 Lane 4: HB09-7 Lane 5: HB09-8 Lane 6: HB09-11 Lane 7: HB08-19 Lane 8: HB08-20

  4. A p-SMAD SMAD-1 HO Sample 2 HO Sample 1 B 2.5 2 pSmad/Smad1 1.5 1 0.5 0 HO Sample 1 HO Sample 2 Control C c.270 C>T c.270 C c.690 G>A c.690 G c.270 C>T HO sample 1: c.270 C>T; c.67-236 A>a/g; c.690 G>A dG= -446.17kcal/mol HO sample 2: c.270 C>T; c.67-237 delT dG= -446.17kcal/mol Control sample (no polymorphisms) dG= -444.97kcal/mol