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Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)

Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011). Presentation Content. Introduction Work plan Foundation. Introduction Damage of Huanglongbing (HLB). Citrus Huanglongbing. Damage of Huanglongbing. Introduction Vector of Huanglongbing.

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Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)

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  1. Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)

  2. Presentation Content • Introduction • Work plan • Foundation

  3. Introduction Damage of Huanglongbing (HLB) Citrus Huanglongbing Damage of Huanglongbing

  4. Introduction Vector of Huanglongbing Diaphorina citri(ACP) Transmitting HLB

  5. Introduction Control of ACP Controlling ACP is a main and effective approach to manage HLB

  6. Introduction Advancement of ACP Control Chemical control • Conventional approach • Usually undesirable • Many side effects

  7. Introduction Advancement of ACP Control Biological control • Environmentally friendly • Few biological agents studied • No biological agents used in practice of ACP control

  8. Introduction Potential of microbial for bio-control of ACP • Microbial are resources of biological agents • against pest insects Biological pesticides Gene resource for GMP

  9. Introduction Potential of microbial for bio-control of ACP 2. Reported microbial against ACP Cephalosphorium lecanii Zimm Isaria fumosorosea Wize

  10. Work plan Objective Find new microbial for ACP management

  11. Work plan Content • Occurrence of ACP microbial parasites in field • Isolation and reconfirmation of ACP microbial parasites • Identification of ACP microbial parasites • Biology of ACP microbial parasites • Production technology of ACP microbial parasites • Evaluation of the efficiency of ACP microbial parasites

  12. Work plan • Occurrence of ACP microbial parasites in field 1.1 Population dynamic of ACP 1.2 Occurrence of ACP parasites 1.3 Contribute of ACP parasites to regulating ACP population 1.4 Collection of ACP parasites from field Timeline: 2010/04-2011/04

  13. Work plan 2. Isolation and reconfirmation of ACP microbial parasites 2.1 Rearing ACP for bioassay 2.2 Isolating and reconfirming microbial pathogenic against ACP 2.2.1 Microbial from infected ACP 2.2.2 Microbial inhabiting on plant surface 2.2.3 Microbial endophyte inner plant 2.2.4 Microbial from soil Timeline: 2010/04-2010/12

  14. Work plan 3. Identification of ACP microbial parasites 3.1 Fungi pathogenic against ACP 3.2 Bacteria pathogenic against ACP 3.3 Virus pathogenic against ACP (Send to taxonomist for identification) Timeline: 2010/04-2010/12

  15. Work plan 4. Biology of ACP microbial parasites 4.1 Climatic condition for microbial growth 4.2 Nutrition condition for microbial growth 4.3 Effects of climatic condition on microbial pathogenicity 4.4 Effects of nutrition medium on microbial pathogenicity Timeline: 2010/08-2011/03

  16. Work plan 5. Production technology of ACP microbial parasites 5.1 Fermentation condition Medium formula, temperature, inoculum, rotation rate, etc. 5.2 Formation Timeline: 2010/04-2011/06

  17. Work plan 6. Evaluation of the efficiency of ACP microbial parasites 6.1 Greenhouse experiments 6.2 Field experiments Timeline: 2010/06-2011/12

  18. Work plan Deliverable results • Report of population dynamic of ACP and the natural occurrence of its microbial parasites • Isolation of 2-3 microbial strains against ACP

  19. Work plan Budget for this year Personel (salary etc): 7400 USD Materials: 3000 USD Travels: 4000 USD Eletricity energy: 1600 USD Collaborator: 3000 USD Overhead: 1000 USD Sum: 20000 USD

  20. Work plan Budget Narrative 1 Personal: We need to pay our students and scientists working on the project Tow graduate students, 150 USD/month, 10 month for work, total 3000 USD tow months of a senior scientist, 1000 USD/month, total 2000 USD One full time research assistant, 200 USD/month, 12 month for work, total 2400 USD Sum 7400 USD

  21. Work plan Budget Narrative 2 Materials: Purchasing citrus seedlings and culturing pots to rear Asian Citrus Psyllid, 1500 USD Purchasing medium, Petri discs for culturing microbials, 1500 USD Sum 3000 USD Travels: Travel to citrus orchards in different regions for investigation and sampling, 4000 USD Spending of eletricity energy: 1600 USD

  22. Work plan Budget Narrative 3 Collaborator: Payment for citrus farmer for their information and offering citrus orchard for experiment, 1000 USD Identification of entomopathogens of Asian Citrus Psyllid, 2000 USD Sum 3000 USD Overhead: The fund for the institute to maintain the facilities on good condition for conducting experiments, 1000 USD

  23. Foundation • Long history of study on Huanglongbing and • its vector • Morphology of HLB Pathogen • Pathology and epidemiology of HLB • Biology, ecology and control of ACP

  24. Foundation • Long history of study on Huanglongbing and • its vector • Morphology of HLB Pathogen • Pathology and epidemiology of HLB • Biology, ecology and control of ACP

  25. Foundation • Profound research and utilization of microbial • 2.1 Collection center of microbial resource >5000 soil samples from different regions over the world >10000 Bacillus strains

  26. Foundation • Profound research and utilization of microbial • 2.2 Utilization of microbial A R&D Center Pesticide Feedstuff Bacillus Fertilizer Clarifier Preservative

  27. Foundation • Profound research and utilization of microbial • 2.3 Research projects • 2.3.1 Study on Bio-insecticide BtA

  28. Foundation • Profound research and utilization of microbial • 2.3 Research projects • 2.3.2 Study on Bio-bactericide strain ANTI-8098A • against Ralstonia solanacearum of vegetables

  29. Foundation • Profound research and utilization of microbial • 2.3 Research projects • 2.3.3 Study on Bio-preservative for fruit Long’an

  30. Bio-preservative for fruit Long’an

  31. Foundation 3. Condition for research

  32. Foundation 4. Research group

  33. Foundation 4. Publications

  34. Thank you for your attention ! Wellcome to China !

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