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DES1 UBQ 10 Wt des1-2 Wt des1-1 9.4 7.1 4.3 3.1 LBb1 (a) T-DNA SALK_103855 (des1-1) GW-CSL-F DES1 GW-CSL-R RATM13-2715-1_G (des1-2) Ds-trans DS3-4 (b) DES1 UBQ 10 Wt des1-1 (c) Figure S4. A, Intron-exon organization of the DES1 gene (At5g28030) in the SALK__103855 and RATM13-2715-1_G insertion mutants, named des1-1 and des1-2, respectively. LBb1, DS3-4, F and R show the locations and directions of primers used in the screening for mutant plants. The gene structure and T-DNA region are not drawn to scale. B, RT-PCR analysis of the mutant plants. RNA samples were prepared from the leaves of two-week-old plants, and primers specific for DES1 and constitutive UBQ10 transcripts were used. The des1-1 and des1-2 mutants are null mutants. C, Southern blot analysis of the des1-1 mutant plant. Genomic DNA prepared from the leaves was digested with XbaI and hybridized with a T-DNA probe. The des1-1 mutant plants contain a unique T-DNA insertion.