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MS Title Page: should include the title, name of each author, and the institution at which the work was performed. Text should be centered. DWA3, an Arabidopsis DWD protein, acts as a negative regulator in ABA signal transduction

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Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • Title Page: should include the title, name of each author, and the institution at which the work was performed.

  • Text should be centered.

  • DWA3, an Arabidopsis DWD protein, acts as a negative regulator in ABA signal

  • transduction

  • Jae-Hoon Lee, a William Terzaghi,a,b and Xing Wang Deng a,*

  • a Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104, U.S.A.

  • b Department of Biology, Wilkes University, Wilkes-Barre, PA 18766, U.S.A.

  • * Corresponding author, Fax: +1 203 432 3204

  • E-mail address: xingwang.deng@yale.edu

  • Keywords: CUL4-based E3 ligase; DWD; ABA; negative regulator; signal transduction pathway; Arabidopsis


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • DWD proteins have been reported as substrate receptors for cullin–RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of DWD genes for abscisic acid (ABA) responses we obtained several candidates which exhibited ABA-hypersensitivity and one was named DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the CRL4 components DDB1 and CUL4, indicating that DWA3 may function as a substrate receptor for CRL4. ABA-inducible transcription factors (ABI5 and AtMYC2) and their downstream genes were hyper-induced by ABA in dwa3. Taken together, we suggest DWA3 is a negative regulator of ABA responses and may be involved in protein degradation mediated by CRL4.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • DWD proteins have been reported as substrate receptors for cullin–RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of DWD genes for abscisic acid (ABA) responses we obtained several candidates which exhibited ABA-hypersensitivity and one was named DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the CRL4 components DDB1 and CUL4, indicating that DWA3 may function as a substrate receptor for CRL4. ABA-inducible transcription factors (ABI5 and AtMYC2) and their downstream genes were hyper-induced by ABA in dwa3. Taken together, we suggest DWA3 is a negative regulator of ABA responses and may be involved in protein degradation mediated by CRL4.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • should have 1 or 2 sentences of Introduction

  • DWD proteins have been reported as substrate receptors for cullin–RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of DWD genes for abscisic acid (ABA) responses we obtained several candidates which exhibited ABA-hypersensitivity and one was named DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the CRL4 components DDB1 and CUL4, indicating that DWA3 may function as a substrate receptor for CRL4. ABA-inducible transcription factors (ABI5 and AtMYC2) and their downstream genes were hyper-induced by ABA in dwa3. Taken together, we suggest DWA3 is a negative regulator of ABA responses and may be involved in protein degradation mediated by CRL4.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • should have 1 or 2 sentences of Introduction

  • 1- 2 sentences of Materials and Methods

  • DWD proteins have been reported as substrate receptors for cullin–RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of DWD genes for abscisic acid (ABA) responses we obtained several candidates which exhibited ABA-hypersensitivity and one was named DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the CRL4 components DDB1 and CUL4, indicating that DWA3 may function as a substrate receptor for CRL4. ABA-inducible transcription factors (ABI5 and AtMYC2) and their downstream genes were hyper-induced by ABA in dwa3. Taken together, we suggest DWA3 is a negative regulator of ABA responses and may be involved in protein degradation mediated by CRL4.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • should have 1 or 2 sentences of Introduction

  • 1- 2 sentences of Materials and Methods

  • 3-4 sentences of Results, including quantitative data


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • should have 1 or 2 sentences of Introduction

  • 1- 2 sentences of Materials and Methods

  • 3-4 sentences of Results, including quantitative data

  • 1-2 sentences of Discussion


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 2. Abstract: summarizes the paper in 250 words or less.

  • mini-papers that are often published separately

  • should have 1 or 2 sentences of Introduction

  • 1- 2 sentences of Materials and Methods

  • 3-4 sentences of Results, including quantitative data

  • 1-2 sentences of Discussion

  • rarely cite references. If they do, full citation must be included.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 3. Introduction: explains why the experiment was done.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 3. Introduction: explains why the experiment was done.

  • provides enough detail about what was previously known to explain what the outstanding questions are


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 3. Introduction: explains why the experiment was done.

  • provides enough detail about what was previously known to explain what the outstanding questions are

  • specifically states the hypothesis that was tested.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 3. Introduction: explains why the experiment was done.

  • provides enough detail about what was previously known to explain what the outstanding questions are

  • specifically states the hypothesis that was tested.

  • I write it last, to guide readers to my results


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 4. Materials and Methods: provide sufficient detail that another biologist could repeat your experiment.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 4. Materials and Methods: provide sufficient detail that another biologist could repeat your experiment.

  • list the organisms used (including the latin binomial) the names of the reagents, procedures followed, etc


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 4. Materials and Methods: provide sufficient detail that another biologist could repeat your experiment.

  • list the organisms used (including the latin binomial) the names of the reagents, procedures followed, etc

  • do not give the recipe for each reagent.

    • Instead, cite the reference from which the recipe was obtained.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 4. Materials and Methods: provide sufficient detail that another biologist could repeat your experiment.

  • list the organisms used (including the latin binomial) the names of the reagents, procedures followed, etc

  • do not give the recipe for each reagent.

  • Do cite the manufacturers of esoteric reagents or equipment


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 4. Materials and Methods: provide sufficient detail that another biologist could repeat your experiment.

  • list the organisms used (including the latin binomial) the names of the reagents, procedures followed, etc

  • do not give the recipe for each reagent.

  • Do cite the manufacturers of esoteric reagents or equipment

  • Both Materials and Methods and Results should be written in the past tense. Routine calculations are not described here, unless they are done in an unusual way.


Ms 2 abstract summarizes the paper in 250 words or less

MS

5. Results: devote one paragraph to each figure or table


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods

  • third sentence states where the results are presented


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods

  • third sentence states where the results are presented

  • remaining sentences point out the key features.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods

  • third sentence states where the results are presented

  • remaining sentences point out the key features.

  • Results can be presented as figures or tables, which should be presented on separate pages attached after the “literature cited”


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods

  • third sentence states where the results are presented

  • remaining sentences point out the key features.

  • Results can be presented as figures or tables, which should be presented on separate pages attached after the “literature cited”

  • each figure or table should have its own title and caption


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 5. Results: devote one paragraph to each figure or table

  • start with a sentence explaining the purpose of the expt

  • second sentence should summarize the methods

  • third sentence states where the results are presented

  • remaining sentences point out the key features.

  • Results can be presented as figures or tables, which should be presented on separate pages attached after the “literature cited”

  • each figure or table should have its own title and caption

  • caption gives sufficient information that a reader can figure out what it is about without reading the text. i.e. summarizes M&M & identifies panels, symbols, etc


Ms 2 abstract summarizes the paper in 250 words or less

MS

6. Discussion: one paragraph per figure or table + a global discussion at end


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 6. Discussion: one paragraph per figure or table + a global discussion at end

  • First sentence summarizes results


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 6. Discussion: one paragraph per figure or table + a global discussion at end

  • First sentence summarizes results

  • Remaining sentences explain them, and may propose ways to test these explanations


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 6. Discussion: one paragraph per figure or table + a global discussion at end

  • First sentence summarizes results

  • Remaining sentences explain them, and may propose ways to test these explanations

  • Last paragraph discusses global implications: now that we know this, how does it change our world?


Ms 2 abstract summarizes the paper in 250 words or less

MS

7. Literature cited: all citations listed in the text should be listed at the end of the paper.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 7. Literature cited: all citations listed in the text should be listed at the end of the paper.

  • Formats for citing and listing references vary among journals.


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 7. Literature cited: all citations listed in the text should be listed at the end of the paper.

  • Formats for citing and listing references vary among journals.

  • Use the format (Smith, 2008) in the text


Ms 2 abstract summarizes the paper in 250 words or less

  • MS

  • 7. Literature cited: all citations listed in the text should be listed at the end of the paper.

  • Formats for citing and listing references vary among journals.

  • Use the format (Smith, 2008) in the text

  • Use this format in the “Literature Cited:” Smith, E.J. (2008) BRF3 encodes a novel ubiquitin ligase. Molecular Plant3: 345-361


Ms 2 abstract summarizes the paper in 250 words or less

Manuscript Draft Grading Checklist

1) Abstract (6 pts)

Were all elements (I, M&M, R & D) present?

Were all elements adequately explained?

Was all information relevant?

Was all information clearly and succinctly explained?

2) Introduction (6 pts)

Was the hypothesis (or purpose) clearly stated?

Was adequate background information provided?

Was all information relevant?

Was all information clearly explained?

3) Materials and Methods (4 pts)

Were all procedures clearly and accurately explained?

Was all information relevant?


Ms 2 abstract summarizes the paper in 250 words or less

4) Results (10 pts)

Was there a separate paragraph for each expt?

Did the first sentence of each para state the purpose?

Did the second sentence summarize the methods?

Did the third sentence state where the results are?

Did the remaining sentences explain the figure/table and point out key results?

Did figures and tables do the job?

Titles and captions?

5) Discussion (8 pts)

Were key results summarized?

Were key results discussed?

Were further experiments proposed?

Were broader implications discussed?


Ms 2 abstract summarizes the paper in 250 words or less

6) Literature cited (2 pts)

Were references used correctly in the text?

Were all citations made in text listed in correct format?

Were any references not cited in the text?

7) Writing (4 pts)

Organization

Clarity

Conciseness

Grammar and spelling