Laboratory Procedures. Bacteria. Culturing Bacteria. Culturing: To introduce a specific microorganism into a solid or liquid broth in order to grow that microorganism for laboratory testing. Four steps: Swabbing the surface/patient you want to culture.
1. The streaking tool is held in open flame to sterilize, then dipped into a colony or culture.
2. The culture is moved in a back-and-forth motion across the top of the plate.
3. The plate is turned 90˚ and a sterile loop is used to streak down from one corner, only touching the previously streaked region once, then continuing up and down the far side of the plate.
4. The plate is turned 90˚ again, and a sterile loop is used to repeat the streaking process.