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Arabidopsis Thaliana Gene AT5G58610 PowerPoint Presentation
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Arabidopsis Thaliana Gene AT5G58610

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Arabidopsis Thaliana Gene AT5G58610 - PowerPoint PPT Presentation

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Arabidopsis Thaliana Gene AT5G58610

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  1. Nancy Gallardo HC70AL Arabidopsis Thaliana GeneAT5G58610

  2. What Protein Does AT5G58610 Code for? • Regulates gene expression • 1065 amino acids PHD-Type Zinc Finger Transcription Factor

  3. What's Around Gene AT5G58610 ? AT5G58600 5' 3' 3' 5' 3' 5' 5' 3' AT5G58600 AT5G8610 AT5G58620 AT5G58620 5' 3' 5' 3' 3' 5' 3' 5' 1045bp 2286bp 23703997-23708279

  4. What is the Structure of AT5G58610 ? 3' 5' 5' 3' Exon Exon Exon Exon Exon Exon Exon Exon Exon • Chromosome 5 • 4283 base pairs • Reverse Orientation • 9 Exons and 8 Introns

  5. Expected T-DNA InsertionSalk_041367 LBb1-150bp Expected Sizes: WT= 908bp T-DNA= 696bp T-DNA Rv- 20bp 3' 5' Intron 2 Intron 1 546bp 362bp Exon 1 Fw- 20bp

  6. What Were the Genotyping Results ? WT= 908 T-DNA=696 PCR Genotyping AT5G58610 700bp 1% agarose, 105 Volts, 04/15/2008, 1.5Hour Run

  7. Do these results agree with the RT-PCR results?

  8. Where is AT5G58610 Active? RT-PCR AT5G58610 1% Agarose 1 Hour Run 70 Volts 04/24/2008

  9. Arabidopsis Thaliana GeneAT4G33280

  10. What Protein Does My Gene Code for? • B3-Domain Transcription Factor Families • Regulate gene expression • 3 families in Arabidopsis Thaliana • 337 amino acids

  11. What's Around Gene AT4G33280 ? 5' 3' 3' 3' 5' AT4G33270 AT4G33280 AT4G33290 5' 5' 3' 3' 561bp 217bp 5' 16047358-16049359

  12. What is the Structure of AT4G33280? 3' 5' 5' 3' Exon Exon Exon Exon Exon UTR UTR Intron Intron Intron Intron • Chromosome 4 • 2002 Base pairs • Reverse Orientation • 5 Exons, 4 Introns, 2 UTRs

  13. Expected T-DNA Insertion Salk_072801 Expected Sizes: WT= 402bp T-DNA= 292bp LBb1-150bp T-DNA Rv-20bp 5' 3' AT4G32270 260Bp 142bp Intron 4 Exon 5 Fw-23bp

  14. What Were the Plants' Genotypes ? Expected T-DNA=292bp Expected Wild Type=402bp PCR Genotyping AT4G33280 Wild Type= 25 Homo T-DNA= 0 Hemi T-DNA= 0 1% agarose 1 Hour 05/15/2008 105 Volts

  15. Carrying Out More Genotyping PCR Genotyping AT4G33280 1% agarose 05/29/08 1Hr. Run 105 Volts

  16. Genotyping With Controls PCR Genotyping of Plants: 7, 17, 26, 33 1% Gel 05/30/08 1 Hr.Run 105 Volts

  17. AT4G33280's Activity RT-PCR AT4G33280 5:34-7:12 pm 1.5% Agarose 05/20/2008 105 Volts Expected Product= 254bp

  18. Cloning the Upstream Region Colony Digestion pENTR+ Gene Vector Promoter Non-Recombinant 2580bp 262bp Recombinant 2842bp 1% Agarose, 1 Hour Run, 1% agarose, 105 Volts

  19. Nomarski

  20. THANKS TO: Dr. Goldberg Anhthu Bui Bekah Charney Daisy Robinton Brandon Le Tomokazu Kawashima