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DNA Sample Management Solutions for Research Projects

Explore comprehensive DNA extraction, storage, and genotyping services at the DNA Resources Core. Secure database system and HIPAA compliance ensure data privacy. Tailored genotyping services, including SNP assays. Manage your samples with us today!

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DNA Sample Management Solutions for Research Projects

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  1. The DNA Resources Core518 Light Hall • Manager: Cara Sutcliffe 6-2744 cara@chgr.mc.vanderbilt.edu • Faculty Director: Doug Mortlock 6-1671 mortlock@chgr.mc.vanderbilt.edu

  2. You’re collecting patient samples …Now what? • DNA extraction from blood or buccal swabs • Storage of extracted DNA • Secure, but practical database system for managing samples • DNA retrieval at a later time, for genotyping samples of interest • SNP genotyping service appropriate to your scale and needs • Compliant with HIPAA

  3. They are still your samples! • Control of samples belongs to the investigator • Password-protected, Web-based database • Non-authorized personnel cannot access sample information or place requisitions for DNA aliquots • “Outsourcing” extraction, storage and organization of DNA samples.

  4. DNA extraction from blood • Gentra Puregene reagent system • ~350 µg DNA from 10 ml blood • Autopure machine • Bar coded

  5. DNA archiving • DNA tubes: ID #, bar coded • Puregene reagent system: Purified DNA is stored dissolved in TE at 4˚. Stable for >10 years

  6. DNA extraction: standard service • DNA Extraction from Whole Blood For 10mls (or 20mls) of blood, services include: • logging in and maintenance of sample information in our database, • DNA extraction, • archiving backup blood spot cards and frozen blood aliquots, • DNA quantitation by fluorometry, • one DNA requisition, • storage of extracted DNA for 5 years.

  7. Additional services • Lymphoblast immortalization & cell line banking • Whole-genome amplification for depleted DNAs • Real-time PCR

  8. Genotyping services • ABI 7900HT machine (Dan Roden/Pharmacogenetics of Arrythmia Therapy) • TaqMan SNP assays Typically >99% accurate • Assays-on-demand: Pre-validated SNP assays by ABI • Assays-by-design: For “custom” SNPs; need to be validated before use

  9. Genotyping services • ABI 7900HT machine (Dan Roden/Pharmacogenetics of Arrythmia Therapy) • TaqMan SNP assays Typically >99% accurate • Assays-on-demand: Pre-validated SNP assays by ABI • Assays-by-design: For “custom” SNPs; need to be validated before use

  10. Genotyping services • Service models using the ABI 7900HT genotyping platform: 1. “Full service”: Customer selects Assays-on-demand; core staff set up TaqMan PCR assays in 384 well plates, and scan plates on 7900HT to collect genotypes. ($400 setup + $0.80/genotype) 2. As 1, and core staff assist with designing custom Assays-by-design (inquire) 3. Customer obtains Assays-by-design; core staff perform PCR and scan plates. ($95 setup + $0.80/genotype) 4. Customer is responsible for setting up PCR and scanning plates ($10/plate).

  11. The DNA Resources Core518 Light Hall • Manager: Cara Sutcliffe 6-2744 cara@chgr.mc.vanderbilt.edu • Faculty Director: Doug Mortlock 6-1671 mortlock@chgr.mc.vanderbilt.edu

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